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Literature summary extracted from

  • Ahn, S.J.; Kim, N.Y.; Seo, J.S.; Je, J.E.; Sung, J.H.; Lee, S.H.; Kim, M.S.; Kim, J.K.; Chung, J.K.; Lee, H.H.
    Molecular cloning, mRNA expression and enzymatic characterization of cathepsin F from olive flounder (Paralichthys olivaceus) (2009), Comp. Biochem. Physiol. B, 154, 211-220.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.41 Brij 35 273.6% relative activity at 0.01% (v/v) Paralichthys olivaceus
3.4.22.41 Triton X-100 310.29% relative activity at 0.01% (v/v) Paralichthys olivaceus
3.4.22.41 Tween 20 282.7% relative activity at 0.01% (v/v) Paralichthys olivaceus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.41 expressed in Escherichia coli DH5alphaMCR cells using the pGEX-4 T-1 expression vector system Paralichthys olivaceus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.41 1,10-phenanthroline 15.28% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.41 antipain complete inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.41 Aprotinin 36.94% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.41 chymostatin 35.42% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.41 Co2+ 6.85% residual activity at 5 mM Paralichthys olivaceus
3.4.22.41 Cu2+ 11.6% residual activity at 5 mM Paralichthys olivaceus
3.4.22.41 E-64 56.3% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.41 EDTA 6.52% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.41 EGTA 4.72% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.41 Hg2+ 0.81% residual activity at 5 mM Paralichthys olivaceus
3.4.22.41 leupeptin complete inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.41 additional information not inhibited by pepstatin A Paralichthys olivaceus
3.4.22.41 NEM 44.59% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.41 phenylmethylsufonyl fluoride 13.22% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.41 SDS complete inhibition at 0.05% (w/v) Paralichthys olivaceus
3.4.22.41 Zn2+ 5.94% residual activity at 1 mM Paralichthys olivaceus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.41 Ca2+ 149.95% relative activity at 1 mM Paralichthys olivaceus
3.4.22.41 K+ 143.7% relative activity at 1 mM Paralichthys olivaceus
3.4.22.41 Mg2+ 133.38% relative activity at 1 mM Paralichthys olivaceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.41 50000
-
 recombinant His-tag fusion protein, SDS-APGE Paralichthys olivaceus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.41 Paralichthys olivaceus B2Z446 olive flounder
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.41 glutathione-Sepharose column chromatography Paralichthys olivaceus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.41 brain high expression Paralichthys olivaceus
-
3.4.22.41 epidermal mucus immunostaining using anti-PoCtF antibody was strongest on the epidermal mucus in the fin Paralichthys olivaceus
-
3.4.22.41 esophagus high expression Paralichthys olivaceus
-
3.4.22.41 eye high expression Paralichthys olivaceus
-
3.4.22.41 gill
-
 Paralichthys olivaceus
-
3.4.22.41 head kidney high expression Paralichthys olivaceus
-
3.4.22.41 heart high expression Paralichthys olivaceus
-
3.4.22.41 intestine
-
 Paralichthys olivaceus
-
3.4.22.41 kidney high expression Paralichthys olivaceus
-
3.4.22.41 liver high expression Paralichthys olivaceus
-
3.4.22.41 additional information ubiquitous expression throughout the entirety of healthy tissues Paralichthys olivaceus
-
3.4.22.41 muscle
-
 Paralichthys olivaceus
-
3.4.22.41 pyloric cecum
-
 Paralichthys olivaceus
-
3.4.22.41 spleen high expression Paralichthys olivaceus
-
3.4.22.41 stomach
-
 Paralichthys olivaceus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.41 benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Paralichthys olivaceus benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.41 Gelatin + H2O purified CtF is capable of hydrolyzing 0.1% gelatin at pH 7.5 Paralichthys olivaceus ?
-
 ?
3.4.22.41 additional information the enzyme is not able to hydrolyze protein substrates such as azocasein, fibrinogen, and bovine serum albumin Paralichthys olivaceus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.41 CtF
-
 Paralichthys olivaceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.41 7.5
-
-
 Paralichthys olivaceus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.41 6.5 8.5
-
 Paralichthys olivaceus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.41 5
-
 activity is greatly decreased at pH 5 (lysosomal pH) and below Paralichthys olivaceus

Expression

EC Number Organism Comment Expression
3.4.22.41 Paralichthys olivaceus CtF expressions increases significantly in gill at 3 h post-injection with lipopolysaccharide up