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Literature summary extracted from

  • Je, J.E.; Ahn, S.J.; Kim, N.Y.; Seo, J.S.; Kim, M.S.; Park, N.G.; Kim, J.K.; Chung, J.K.; Lee, H.H.
    Molecular cloning, expression analysis and enzymatic characterization of cathepsin K from olive flounder (Paralichthys olivaceus) (2009), Comp. Biochem. Physiol. A, 154, 474-485.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.38 Brij 35 245.48% relative activity at 0.01% (v/v) Paralichthys olivaceus
3.4.22.38 SDS 135.84% relative activity at 0.01% (w/v) Paralichthys olivaceus
3.4.22.38 Triton X-100 218.51% relative activity at 0.05% (v/v) Paralichthys olivaceus
3.4.22.38 Tween 20 218.04% relative activity at 0.05% (v/v) Paralichthys olivaceus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.38 expressed in Escherichia coli strain DH5alphaMCR using the pGEX-4T-1 expression vector system Paralichthys olivaceus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.38 antipain 98.91% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.38 Aprotinin 25.08% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.38 chymostatin 44.58% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.38 Co2+ 5.09% residual activity at 5 mM Paralichthys olivaceus
3.4.22.38 Cu2+ 24.86% residual activity at 5 mM Paralichthys olivaceus
3.4.22.38 E-64 60.15% inhibition at 0.5 mM Paralichthys olivaceus
3.4.22.38 EDTA 3.72% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.38 Hg2+ 25.27% residual activity at 5 mM Paralichthys olivaceus
3.4.22.38 leupeptin complete inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.38 additional information not inhibited by EGTA and 1,10-phenanthroline Paralichthys olivaceus
3.4.22.38 pepstatin A 17.29% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.38 phenylmethylsulfonyl fluoride 53.68% inhibition at 0.1 mM Paralichthys olivaceus
3.4.22.38 SDS 77.28% residual activity at 0.05% (w/v) Paralichthys olivaceus
3.4.22.38 Zn2+ 47.5% residual activity at 5 mM Paralichthys olivaceus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.38 Ca2+ 390.04% relative activity at 1 mM Paralichthys olivaceus
3.4.22.38 K+ 332.4%7 relative activity at 1 mM Paralichthys olivaceus
3.4.22.38 Mg2+ 125.02% relative activity at 5 mM Paralichthys olivaceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.38 50000
-
 His6-tagged fusion protein, SDS-PAGE Paralichthys olivaceus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.38 collagen I + H2O Paralichthys olivaceus
-
 ?
-
 ?
3.4.22.38 collagen II + H2O Paralichthys olivaceus
-
 ?
-
 ?
3.4.22.38 collagen III + H2O Paralichthys olivaceus
-
 ?
-
 ?
3.4.22.38 Collagen IV + H2O Paralichthys olivaceus
-
 ?
-
 ?
3.4.22.38 collagen VI + H2O Paralichthys olivaceus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.38 Paralichthys olivaceus B2Z447 olive flounder
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.38 glutathione-Sepharose column chromatography Paralichthys olivaceus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.38 bone
-
 Paralichthys olivaceus
-
3.4.22.38 bone marrow high levels of expression Paralichthys olivaceus
-
3.4.22.38 brain
-
 Paralichthys olivaceus
-
3.4.22.38 esophagus
-
 Paralichthys olivaceus
-
3.4.22.38 eye
-
 Paralichthys olivaceus
-
3.4.22.38 gill
-
 Paralichthys olivaceus
-
3.4.22.38 head kidney
-
 Paralichthys olivaceus
-
3.4.22.38 heart
-
 Paralichthys olivaceus
-
3.4.22.38 intestine
-
 Paralichthys olivaceus
-
3.4.22.38 liver
-
 Paralichthys olivaceus
-
3.4.22.38 muscle
-
 Paralichthys olivaceus
-
3.4.22.38 pyloric cecum
-
 Paralichthys olivaceus
-
3.4.22.38 spleen high levels of expression Paralichthys olivaceus
-
3.4.22.38 stomach
-
 Paralichthys olivaceus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.38 Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 31.29% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.22.38 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O 47.92% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.38 benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O 89.78% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.38 benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O 100% activity Paralichthys olivaceus benzyloxycarbonyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.38 benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O 23.91% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus benzyloxycarbonyl-Leu-Leu-Glu + 7-amino-4-methylcoumarin
-
 ?
3.4.22.38 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O 71.17% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.38 collagen I + H2O
-
 Paralichthys olivaceus ?
-
 ?
3.4.22.38 collagen II + H2O
-
 Paralichthys olivaceus ?
-
 ?
3.4.22.38 collagen III + H2O
-
 Paralichthys olivaceus ?
-
 ?
3.4.22.38 Collagen IV + H2O
-
 Paralichthys olivaceus ?
-
 ?
3.4.22.38 collagen VI + H2O
-
 Paralichthys olivaceus ?
-
 ?
3.4.22.38 additional information cathepsin K is not able to hydrolyze protein substrates such as azocasein, fibrinogen, and bovine serum albumin Paralichthys olivaceus ?
-
 ?
3.4.22.38 N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O 24.13% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus N-succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.22.38 N-succinyl-Ile-Ala-7-amido-4-methylcoumarin + H2O 17.34% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus N-succinyl-Ile-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.22.38 N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O 25.03% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.22.38 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O 31.57% activity compared to benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin Paralichthys olivaceus N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.38 Ctk
-
 Paralichthys olivaceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.38 8
-
-
 Paralichthys olivaceus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.38 6.5 9
-
 Paralichthys olivaceus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.38 6
-
 the activity of cathepsin K is profoundly reduced at pH values of 6.0 (lysosomal pH) and below Paralichthys olivaceus

Expression

EC Number Organism Comment Expression
3.4.22.38 Paralichthys olivaceus CtK expression is significantly increased in the muscle and gill at 3-24 h post-injection with bacterial lipopolysaccharide up

General Information

EC Number General Information Comment Organism
3.4.22.38 physiological function cathepsin K may play a role in the immune system of fish skin and muscle, in addition to its principal bone-specific function as a collagenolytic enzyme Paralichthys olivaceus