BRENDA - Enzyme Database

Effect of synthetic and natural phospholipids on N-acylphosphatidylethanolamine-hydrolyzing phospholipase D activity

Petersen, G.; Pedersen, A.H.; Pickering, D.S.; Begtrup, M.; Hansen, H.S.; Chem. Phys. Lipids 162, 53-61 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.54
1,2-dihexanoyl-glycero-3-(hexanoylamino)propylphosphonate
i.e. AHP-57, a phosphonic acid, activates 1.9fold at 1 mM
Homo sapiens
3.1.4.54
cardiolipin
from bovine liver, activates by 68% at 1 mM
Homo sapiens
3.1.4.54
methyl-1,2-dihexanoyl-glycero-3-(hexanoylamino)propylphosphonate
i.e. AHP-38, a methylphosphonate, activates 68% at 1 mM
Homo sapiens
3.1.4.54
methyl-1,2-dihexanoyl-glycero-3-(tetradecanoylamino)propylphosphonate
i.e. AHP-36, a methylphosphonate, activates 2fold at 1 mM
Homo sapiens
3.1.4.54
additional information
activator synthesis, overview
Homo sapiens
3.1.4.54
phosphatidylbutanol
activates 81% at 1 mM
Homo sapiens
3.1.4.54
phosphatidylcholine
activates 2.75fold at 1 mM
Homo sapiens
3.1.4.54
phosphatidylethanolamine
activates; activates 5.91fold at 1 mM
Homo sapiens
3.1.4.54
phosphatidylinositol
activates 99% at 1 mM
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.4.54
stable expression of NAPE-PLD in HEK-293 cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.54
1,2-dihexanoyl-glycero-N-(3-(tetradecanoylamino)propyl)phosphoramidate
i.e. AHP-71B, 96.6% inhibition at 1 mM
Homo sapiens
3.1.4.54
N-(2,4-dinitrophenyl)-1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine
1 mM, 35.6% inhibition
Homo sapiens
3.1.4.54
nitrocefin
-
Homo sapiens
3.1.4.54
phosphatidylhexanol
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.54
Zn2+
NAPE-PLD is a zinc metallohydrolase
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.54
N-acylphosphatidylethanolamine + H2O
Homo sapiens
N-acylethanolamines constitute a family of endogenous bioactive lipids that includes arachidonoylethanolamide, i.e. anandamide, palmitoylethanolamide, and oleoylethanolamide. These lipids are formed from their respective N-acylated ethanolamine phospholipid precursor by the action of a phospholipase D enzyme, NAPE-PLD. The bioactive lipids may influence, amongst others: neuroinflammation, food intake, and oocyte implantation
N-acylethanolamine + phosphatidate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.54
Homo sapiens
Q6IQ20
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.54
1,2-dihexadecyl-sn-glycerol-3-phospho-(N-palmitoyl)ethanolamine + H2O
-
708110
Homo sapiens
N-palmitoylethanolamine + 1,2-dihexadecyl-sn-glycerol-3-phosphate
-
-
-
?
3.1.4.54
N-acylphosphatidylethanolamine + H2O
N-acylethanolamines constitute a family of endogenous bioactive lipids that includes arachidonoylethanolamide, i.e. anandamide, palmitoylethanolamide, and oleoylethanolamide. These lipids are formed from their respective N-acylated ethanolamine phospholipid precursor by the action of a phospholipase D enzyme, NAPE-PLD. The bioactive lipids may influence, amongst others: neuroinflammation, food intake, and oocyte implantation
708110
Homo sapiens
N-acylethanolamine + phosphatidate
-
-
-
?
3.1.4.54
N-palmitoyl-1,2-dilauroylphosphatidylethanolamine + H2O
-
708110
Homo sapiens
N-palmitoylethanolamine + 1,2-dilauroyl-sn-glycerol 3-phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.54
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.54
8
-
assay at
Homo sapiens
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.4.54
0.01
-
pH 8.0, 37°C
Homo sapiens
1,2-dihexanoyl-glycero-N-(3-(tetradecanoylamino)propyl)phosphoramidate
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.54
1,2-dihexanoyl-glycero-3-(hexanoylamino)propylphosphonate
i.e. AHP-57, a phosphonic acid, activates 1.9fold at 1 mM
Homo sapiens
3.1.4.54
cardiolipin
from bovine liver, activates by 68% at 1 mM
Homo sapiens
3.1.4.54
methyl-1,2-dihexanoyl-glycero-3-(hexanoylamino)propylphosphonate
i.e. AHP-38, a methylphosphonate, activates 68% at 1 mM
Homo sapiens
3.1.4.54
methyl-1,2-dihexanoyl-glycero-3-(tetradecanoylamino)propylphosphonate
i.e. AHP-36, a methylphosphonate, activates 2fold at 1 mM
Homo sapiens
3.1.4.54
additional information
activator synthesis, overview
Homo sapiens
3.1.4.54
phosphatidylbutanol
activates 81% at 1 mM
Homo sapiens
3.1.4.54
phosphatidylcholine
activates 2.75fold at 1 mM
Homo sapiens
3.1.4.54
phosphatidylethanolamine
activates; activates 5.91fold at 1 mM
Homo sapiens
3.1.4.54
phosphatidylinositol
activates 99% at 1 mM
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.54
stable expression of NAPE-PLD in HEK-293 cells
Homo sapiens
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.4.54
0.01
-
pH 8.0, 37°C
Homo sapiens
1,2-dihexanoyl-glycero-N-(3-(tetradecanoylamino)propyl)phosphoramidate
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.54
1,2-dihexanoyl-glycero-N-(3-(tetradecanoylamino)propyl)phosphoramidate
i.e. AHP-71B, 96.6% inhibition at 1 mM
Homo sapiens
3.1.4.54
N-(2,4-dinitrophenyl)-1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine
1 mM, 35.6% inhibition
Homo sapiens
3.1.4.54
nitrocefin
-
Homo sapiens
3.1.4.54
phosphatidylhexanol
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.54
Zn2+
NAPE-PLD is a zinc metallohydrolase
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.54
N-acylphosphatidylethanolamine + H2O
Homo sapiens
N-acylethanolamines constitute a family of endogenous bioactive lipids that includes arachidonoylethanolamide, i.e. anandamide, palmitoylethanolamide, and oleoylethanolamide. These lipids are formed from their respective N-acylated ethanolamine phospholipid precursor by the action of a phospholipase D enzyme, NAPE-PLD. The bioactive lipids may influence, amongst others: neuroinflammation, food intake, and oocyte implantation
N-acylethanolamine + phosphatidate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.54
1,2-dihexadecyl-sn-glycerol-3-phospho-(N-palmitoyl)ethanolamine + H2O
-
708110
Homo sapiens
N-palmitoylethanolamine + 1,2-dihexadecyl-sn-glycerol-3-phosphate
-
-
-
?
3.1.4.54
N-acylphosphatidylethanolamine + H2O
N-acylethanolamines constitute a family of endogenous bioactive lipids that includes arachidonoylethanolamide, i.e. anandamide, palmitoylethanolamide, and oleoylethanolamide. These lipids are formed from their respective N-acylated ethanolamine phospholipid precursor by the action of a phospholipase D enzyme, NAPE-PLD. The bioactive lipids may influence, amongst others: neuroinflammation, food intake, and oocyte implantation
708110
Homo sapiens
N-acylethanolamine + phosphatidate
-
-
-
?
3.1.4.54
N-palmitoyl-1,2-dilauroylphosphatidylethanolamine + H2O
-
708110
Homo sapiens
N-palmitoylethanolamine + 1,2-dilauroyl-sn-glycerol 3-phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.54
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.54
8
-
assay at
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
3.1.4.54
metabolism
the enzyme catalyzes a step in the biosynthesis of N-acylethanolamines, the biosynthetic route of N-acylethanolamines seems to involve at least three alternative pathways, overview
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.4.54
metabolism
the enzyme catalyzes a step in the biosynthesis of N-acylethanolamines, the biosynthetic route of N-acylethanolamines seems to involve at least three alternative pathways, overview
Homo sapiens