Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Petersen, G.; Pedersen, A.H.; Pickering, D.S.; Begtrup, M.; Hansen, H.S.
    Effect of synthetic and natural phospholipids on N-acylphosphatidylethanolamine-hydrolyzing phospholipase D activity (2009), Chem. Phys. Lipids, 162, 53-61.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.54 1,2-dihexanoyl-glycero-3-(hexanoylamino)propylphosphonate i.e. AHP-57, a phosphonic acid, activates 1.9fold at 1 mM Homo sapiens
3.1.4.54 cardiolipin from bovine liver, activates by 68% at 1 mM Homo sapiens
3.1.4.54 methyl-1,2-dihexanoyl-glycero-3-(hexanoylamino)propylphosphonate i.e. AHP-38, a methylphosphonate, activates 68% at 1 mM Homo sapiens
3.1.4.54 methyl-1,2-dihexanoyl-glycero-3-(tetradecanoylamino)propylphosphonate i.e. AHP-36, a methylphosphonate, activates 2fold at 1 mM Homo sapiens
3.1.4.54 additional information activator synthesis, overview Homo sapiens
3.1.4.54 phosphatidylbutanol activates 81% at 1 mM Homo sapiens
3.1.4.54 phosphatidylcholine activates 2.75fold at 1 mM Homo sapiens
3.1.4.54 phosphatidylethanolamine activates Homo sapiens
3.1.4.54 phosphatidylethanolamine activates 5.91fold at 1 mM Homo sapiens
3.1.4.54 phosphatidylinositol activates 99% at 1 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.54 stable expression of NAPE-PLD in HEK-293 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.54 1,2-dihexanoyl-glycero-N-(3-(tetradecanoylamino)propyl)phosphoramidate i.e. AHP-71B, 96.6% inhibition at 1 mM Homo sapiens
3.1.4.54 N-(2,4-dinitrophenyl)-1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine 1 mM, 35.6% inhibition Homo sapiens
3.1.4.54 nitrocefin
-
 Homo sapiens
3.1.4.54 phosphatidylhexanol
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.54 Zn2+ NAPE-PLD is a zinc metallohydrolase Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.54 N-acylphosphatidylethanolamine + H2O Homo sapiens N-acylethanolamines constitute a family of endogenous bioactive lipids that includes arachidonoylethanolamide, i.e. anandamide, palmitoylethanolamide, and oleoylethanolamide. These lipids are formed from their respective N-acylated ethanolamine phospholipid precursor by the action of a phospholipase D enzyme, NAPE-PLD. The bioactive lipids may influence, amongst others: neuroinflammation, food intake, and oocyte implantation N-acylethanolamine + phosphatidate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.54 Homo sapiens Q6IQ20
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.54 1,2-dihexadecyl-sn-glycerol-3-phospho-(N-palmitoyl)ethanolamine + H2O
-
 Homo sapiens N-palmitoylethanolamine + 1,2-dihexadecyl-sn-glycerol-3-phosphate
-
 ?
3.1.4.54 N-acylphosphatidylethanolamine + H2O N-acylethanolamines constitute a family of endogenous bioactive lipids that includes arachidonoylethanolamide, i.e. anandamide, palmitoylethanolamide, and oleoylethanolamide. These lipids are formed from their respective N-acylated ethanolamine phospholipid precursor by the action of a phospholipase D enzyme, NAPE-PLD. The bioactive lipids may influence, amongst others: neuroinflammation, food intake, and oocyte implantation Homo sapiens N-acylethanolamine + phosphatidate
-
 ?
3.1.4.54 N-palmitoyl-1,2-dilauroylphosphatidylethanolamine + H2O
-
 Homo sapiens N-palmitoylethanolamine + 1,2-dilauroyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.54 NAPE-PLD
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.54 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.54 8
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.4.54 0.01
-
 pH 8.0, 37°C Homo sapiens 1,2-dihexanoyl-glycero-N-(3-(tetradecanoylamino)propyl)phosphoramidate

General Information

EC Number General Information Comment Organism
3.1.4.54 metabolism the enzyme catalyzes a step in the biosynthesis of N-acylethanolamines, the biosynthetic route of N-acylethanolamines seems to involve at least three alternative pathways, overview Homo sapiens