BRENDA - Enzyme Database

The succinate:menaquinone reductase of Bacillus cereus: characterization of the membrane-bound and purified enzyme

Garcia, L.M.; Contreras-Zentella, M.L.; Jaramillo, R.; Benito-Mercade, M.C.; Mendoza-Hernandez, G.; del Arenal, I.P.; Membrillo-Hernandez, J.; Escamilla, J.E.; Can. J. Microbiol. 54, 456-466 (2008)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
2-n-heptyl-4-hydroxyquinolino-N-oxide
-
Bacillus cereus
1.3.5.1
malonate
competitive
Bacillus cereus
1.3.5.4
malonate
competitive
Bacillus cereus
1.3.5.4
additional information
not inhibitory: thenoyltrifluoroacetone, 2-n-heptyl-4-hydroxyquinoline N-oxide
Bacillus cereus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.011
-
decylubiquinone
pH 7.6, 30°C
Bacillus cereus
1.3.5.4
0.33
-
phenazine methosulfate
pH 7.6, 30°C
Bacillus cereus
1.3.5.4
0.64
-
succinate
pH 7.6, 30°C
Bacillus cereus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
membrane
-
Bacillus cereus
16020
-
1.3.5.4
membrane
-
Bacillus cereus
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.1
Bacillus cereus
-
-
-
1.3.5.4
Bacillus cereus
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
1.3.5.4
succinate + a quinone = fumarate + a quinol
reduction of menaquinone-7 is the rate-limiting step in succinate respiration
Bacillus cereus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.5.1
0.0076
-
electron accepotr 2,6-dichlorophenolindophenol, pH 7.6, 30°C
Bacillus cereus
1.3.5.4
0.0076
-
electron acceptor 2,6-dichlorophenolindophenol, pH 7.6, 30°C
Bacillus cereus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
fumarate + reduced decylubiquinone
-
707949
Bacillus cereus
succinate + decylubiquinone
-
-
-
r
1.3.5.1
additional information
enzyme also accetps phenazine methosulfate, reaction of EC 1.3.5.4
707949
Bacillus cereus
?
-
-
-
-
1.3.5.1
succinate + decylubiquinone
-
707949
Bacillus cereus
fumarate + reduced decylubiquinone
-
-
-
r
1.3.5.4
additional information
enzyme also accepts reduced decylubiquinone, reaction of EC 1.3.5.1
707949
Bacillus cereus
?
-
-
-
-
1.3.5.4
succinate + phenazine methosulfate
-
707949
Bacillus cereus
fumarate + reduced phenazine methosulfate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.3.5.1
?
enzyme consists of a 65000 Da flavoprotein SdhA, a 29000 Da iron-sulfur protein SdhB, and a 19000 Da subunit SdhC containing two b-type cytochromes, SDS-PAGE
Bacillus cereus
1.3.5.4
?
enzyme consists of a 65000 Da flavoprotein SdhA, a 29000 Da iron-sulfur protein SdhB, and a 19000 Da subunit SdhC containing two b-type cytochromes, SDS-PAGE
Bacillus cereus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.5.1
30
-
oxidation of succinate, assay at; reduction of fumarate, assay at
Bacillus cereus
1.3.5.4
30
-
oxidation of succinate, assay at; reduction of fumarate, assay at
Bacillus cereus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.5.4
77
-
phenazine methosulfate
pH 7.6, 30°C
Bacillus cereus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
7.8
-
with decylubiquinone-dichlorophenolindophenol as electron acceptor
Bacillus cereus
1.3.5.4
7.8
-
with phenazine methosulfate-dichlorophenolindophenyl as electron acceptor
Bacillus cereus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
FAD
-
Bacillus cereus
1.3.5.1
heme
-
Bacillus cereus
1.3.5.4
FAD
-
Bacillus cereus
1.3.5.4
heme
-
Bacillus cereus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.00062
-
2-n-heptyl-4-hydroxyquinolino-N-oxide
pH 7.6, 30°C
Bacillus cereus
1.3.5.1
0.088
-
malonate
pH 7.6, 30°C
Bacillus cereus
1.3.5.4
0.088
-
malonate
pH 7.6, 30°C
Bacillus cereus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
FAD
-
Bacillus cereus
1.3.5.1
heme
-
Bacillus cereus
1.3.5.4
FAD
-
Bacillus cereus
1.3.5.4
heme
-
Bacillus cereus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
2-n-heptyl-4-hydroxyquinolino-N-oxide
-
Bacillus cereus
1.3.5.1
malonate
competitive
Bacillus cereus
1.3.5.4
malonate
competitive
Bacillus cereus
1.3.5.4
additional information
not inhibitory: thenoyltrifluoroacetone, 2-n-heptyl-4-hydroxyquinoline N-oxide
Bacillus cereus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.5.1
0.00062
-
2-n-heptyl-4-hydroxyquinolino-N-oxide
pH 7.6, 30°C
Bacillus cereus
1.3.5.1
0.088
-
malonate
pH 7.6, 30°C
Bacillus cereus
1.3.5.4
0.088
-
malonate
pH 7.6, 30°C
Bacillus cereus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.011
-
decylubiquinone
pH 7.6, 30°C
Bacillus cereus
1.3.5.4
0.33
-
phenazine methosulfate
pH 7.6, 30°C
Bacillus cereus
1.3.5.4
0.64
-
succinate
pH 7.6, 30°C
Bacillus cereus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
membrane
-
Bacillus cereus
16020
-
1.3.5.4
membrane
-
Bacillus cereus
16020
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.5.1
0.0076
-
electron accepotr 2,6-dichlorophenolindophenol, pH 7.6, 30°C
Bacillus cereus
1.3.5.4
0.0076
-
electron acceptor 2,6-dichlorophenolindophenol, pH 7.6, 30°C
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
fumarate + reduced decylubiquinone
-
707949
Bacillus cereus
succinate + decylubiquinone
-
-
-
r
1.3.5.1
additional information
enzyme also accetps phenazine methosulfate, reaction of EC 1.3.5.4
707949
Bacillus cereus
?
-
-
-
-
1.3.5.1
succinate + decylubiquinone
-
707949
Bacillus cereus
fumarate + reduced decylubiquinone
-
-
-
r
1.3.5.4
additional information
enzyme also accepts reduced decylubiquinone, reaction of EC 1.3.5.1
707949
Bacillus cereus
?
-
-
-
-
1.3.5.4
succinate + phenazine methosulfate
-
707949
Bacillus cereus
fumarate + reduced phenazine methosulfate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.5.1
?
enzyme consists of a 65000 Da flavoprotein SdhA, a 29000 Da iron-sulfur protein SdhB, and a 19000 Da subunit SdhC containing two b-type cytochromes, SDS-PAGE
Bacillus cereus
1.3.5.4
?
enzyme consists of a 65000 Da flavoprotein SdhA, a 29000 Da iron-sulfur protein SdhB, and a 19000 Da subunit SdhC containing two b-type cytochromes, SDS-PAGE
Bacillus cereus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.5.1
30
-
oxidation of succinate, assay at; reduction of fumarate, assay at
Bacillus cereus
1.3.5.4
30
-
oxidation of succinate, assay at; reduction of fumarate, assay at
Bacillus cereus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.5.4
77
-
phenazine methosulfate
pH 7.6, 30°C
Bacillus cereus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
7.8
-
with decylubiquinone-dichlorophenolindophenol as electron acceptor
Bacillus cereus
1.3.5.4
7.8
-
with phenazine methosulfate-dichlorophenolindophenyl as electron acceptor
Bacillus cereus