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Literature summary extracted from

  • Hossain, M.A.; Fujita, M.
    Purification of glyoxalase I from onion bulbs and molecular cloning of its cDNA (2009), Biosci. Biotechnol. Biochem., 73, 2007-2013.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 expressed in Escherichia coli XL1-Blue cells Allium cepa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 21000
-
 calculated from amino acid sequence Allium cepa
4.4.1.5 25000
-
 SDS-PAGE Allium cepa

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Allium cepa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5 ammonium sulfate precipitation, DEAE-cellulose column chromatography, hydroxyapatite column chromatography, and S-hexylglutathione-agarose column chromatography Allium cepa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.5 bulb the highest glyoxalase I concentration is found in mature bulbs Allium cepa
-
4.4.1.5 leaf
-
 Allium cepa
-
4.4.1.5 root
-
 Allium cepa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 1.94
-
 crude extract, at 22°C Allium cepa
4.4.1.5 356
-
 after 184fold purification, at 22°C Allium cepa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 methylglyoxal + glutathione
-
 Allium cepa (R)-S-lactoylglutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 glyoxalase I
-
 Allium cepa

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.5 glutathione
-
 Allium cepa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.4.1.5 Allium cepa calculated from amino acid sequence
-
 5.2

Expression

EC Number Organism Comment Expression
4.4.1.5 Allium cepa glyoxlase I activity decreases in heavy metal stress (exposure to 0.5 mM CdCl2) down
4.4.1.5 Allium cepa low temperature (4°C) stress shows the highest induction (2.6fold) of glyoxalase I activity, followed by salinity (exposure to 400 mM NaCl) and drought (exposure to 10% (v/v) PEG) stress for 7 days up

General Information

EC Number General Information Comment Organism
4.4.1.5 physiological function GLOI plays an important role in the plant abiotic stress response Allium cepa