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Literature summary extracted from

  • Zhang, G.Q.; Dong, X.F.; Wang, Z.H.; Zhang, Q.; Wang, H.X.; Tong, J.M.
    Purification, characterization, and cloning of a novel phytase with low pH optimum and strong proteolysis resistance from Aspergillus ficuum NTG-23 (2010), Biores. Technol., 101, 4125-4131.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 EDTA slight stimulation Aspergillus ficuum
3.1.3.8 EDTA slight stimulation Aspergillus ficuum
3.1.3.8 EDTA slight activation at 1-10 mM Aspergillus ficuum

Application

EC Number Application Comment Organism
3.1.3.8 agriculture the phytase has the potential to be useful as an animal feed supplement Aspergillus ficuum
3.1.3.8 nutrition the phytase has the potential to be useful as an animal feed supplement Aspergillus ficuum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2
-
 Aspergillus ficuum
3.1.3.8
-
 Aspergillus ficuum

General Stability

EC Number General Stability Organism
3.1.3.2 enzyme shows strong resistance toward pepsin and trypsin Aspergillus ficuum
3.1.3.8 enzyme shows strong resistance toward pepsin and trypsin Aspergillus ficuum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 additional information no effect: Ca2+, Mg2+, Mn2+, Zn2+ Aspergillus ficuum
3.1.3.8 additional information no effect: Ca2+, Mg2+, Mn2+, Zn2+ Aspergillus ficuum
3.1.3.8 additional information no activation or inhibition by cations K+, Ca2+, Mg2+, Mn2+, Zn2+, Cd2+, Cu2+, Pb2+, Hg2+, Fe3+, and Al3+ Aspergillus ficuum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 65500
-
 gel filtration Aspergillus ficuum
3.1.3.8 65500
-
 gel filtration Aspergillus ficuum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 phytate + H2O Aspergillus ficuum
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Aspergillus ficuum
-
-
-
3.1.3.2 Aspergillus ficuum NTG-23
-
-
-
3.1.3.8 Aspergillus ficuum
-
-
-
3.1.3.8 Aspergillus ficuum NTG-23
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 native enzyme 71.5fold by anion exchange chromatography, cation exchange chromatography, and gel filtration Aspergillus ficuum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.8 mycelium
-
 Aspergillus ficuum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 150
-
 37°C, pH 2.5 Aspergillus ficuum
3.1.3.8 150
-
 37°C, pH 2.5 Aspergillus ficuum
3.1.3.8 150.1
-
 purified native enzyme, pH 2.5, 37°C Aspergillus ficuum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 2-glycerophosphate + H2O 127% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum glycerate + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O 254% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum 4-nitrophenol + phosphate
-
 ?
3.1.3.2 ADP + H2O 177% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum ? + phosphate
-
 ?
3.1.3.2 AMP + H2O 173% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum adenosine + phosphate
-
 ?
3.1.3.2 ATP + H2O 256% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum ? + phosphate
-
 ?
3.1.3.2 D-fructose 6-phosphate + H2O 228% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum D-fructose + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O 190% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum D-glucose + phosphate
-
 ?
3.1.3.2 myo-inositol hexakisphosphate + H2O sodium salt Aspergillus ficuum 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 2-glycerophosphate + H2O 127% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum glycerate + phosphate
-
 ?
3.1.3.8 4-nitrophenyl phosphate + H2O 254% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum 4-nitrophenol + phosphate
-
 ?
3.1.3.8 4-nitrophenyl phosphate + H2O 254% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 4-nitrophenol + phosphate
-
 ?
3.1.3.8 ADP + H2O 177% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum ? + phosphate
-
 ?
3.1.3.8 AMP + H2O 173% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum adenosine + phosphate
-
 ?
3.1.3.8 AMP + H2O 173% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 adenosine + phosphate
-
 ?
3.1.3.8 ATP + H2O 256% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum ? + phosphate
-
 ?
3.1.3.8 D-fructose 6-phosphate + H2O 228% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum D-fructose + phosphate
-
 ?
3.1.3.8 D-fructose 6-phosphate + H2O 228% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 D-fructose + phosphate
-
 ?
3.1.3.8 D-glucose 6-phosphate + H2O 190% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum D-glucose + phosphate
-
 ?
3.1.3.8 D-glucose 6-phosphate + H2O 190% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum NTG-23 D-glucose + phosphate
-
 ?
3.1.3.8 additional information the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate Aspergillus ficuum ?
-
 ?
3.1.3.8 additional information the enzyme exhibits a broad substrate selectivity, substrate are e.g. ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate, 4-nitrophenyl phosphate, and beta-glycerophosphate Aspergillus ficuum NTG-23 ?
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O sodium salt Aspergillus ficuum myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 phytate + H2O
-
 Aspergillus ficuum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer 1 * 65500, SDS-PAGE Aspergillus ficuum
3.1.3.8 monomer 1 * 65500, SDS-PAGE Aspergillus ficuum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 67
-
-
 Aspergillus ficuum
3.1.3.8 67
-
-
 Aspergillus ficuum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 70
-
 20 min, no loss of activity Aspergillus ficuum
3.1.3.8 60 70 purified native enzyme, stable at 60°C, no obvious loss of activity upon incubation at 70°C for 20 min Aspergillus ficuum
3.1.3.8 70
-
 20 min, no loss of activity Aspergillus ficuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 1.3
-
-
 Aspergillus ficuum
3.1.3.8 1.3
-
-
 Aspergillus ficuum

General Information

EC Number General Information Comment Organism
3.1.3.8 additional information the enzyme shows strong resistance toward pepsin and trypsin Aspergillus ficuum