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Literature summary extracted from

  • Adsul, M.G.; Bastawde, K.B.; Gokhale, D.V.
    Biochemical characterization of two xylanases from yeast Pseudozyma hubeiensis producing only xylooligosaccharides (2009), Biores. Technol., 100, 6488-6495.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide at 100 mM 20% inhibition of PhX20, 30% inhibition of PhX33 Pseudozyma hubeiensis
3.2.1.8 2,4,6-Trinitrobenzenesulfonic acid at 1 mM 90% inhibition of PhX20, 30% inhibition of PhX33 Pseudozyma hubeiensis
3.2.1.8 citraconic anhydride at 50 mM no inhibition of PhX20, 10% inhibition of PhX33 Pseudozyma hubeiensis
3.2.1.8 diethyldicarbonate at 5 mM 95% inhibition of PhX20, 10% inhibition of PhX33 Pseudozyma hubeiensis
3.2.1.8 additional information no or poor inhibition of PhX33 and PhX20 by N-ethylmaleimide, iodoacetate, 2,3-butanedione, and N-acetylimidazole Pseudozyma hubeiensis
3.2.1.8 N-bromosuccinimide complete inhibition of PhX33 and PhX20 at 1 mM Pseudozyma hubeiensis
3.2.1.8 p-chloromercuribenzoate at 50 mM 50% inhibition of PhX20, 40% inhibition of PhX33 Pseudozyma hubeiensis
3.2.1.8 Phenylglyoxal at 10 mM 25% inhibition of PhX20, no inhibition of PhX33 Pseudozyma hubeiensis
3.2.1.8 Phenylmethylsulfonylfluoride at 10 mM 15% inhibition of PhX20, 10% inhibition of PhX33 Pseudozyma hubeiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Km for PhX33 and PhX20 is 7.6 mg/ml and 11.1 mg/ml, respectively using oat-spelt soluble xylan as substrate Pseudozyma hubeiensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 20100
-
 x * 33500, about, PhX33, mass spectrometry, x * 20100, about, PhX20, mass spectrometry Pseudozyma hubeiensis
3.2.1.8 33500
-
 x * 33500, about, PhX33, mass spectrometry, x * 20100, about, PhX20, mass spectrometry Pseudozyma hubeiensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O Pseudozyma hubeiensis
-
 xylooligosaccharides
-
 ?
3.2.1.8 beta-1,4-xylan + H2O Pseudozyma hubeiensis NCIM 3574
-
 xylooligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Pseudozyma hubeiensis
-
 isolated from decaying sandal wood
-
3.2.1.8 Pseudozyma hubeiensis NCIM 3574
-
 isolated from decaying sandal wood
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein PHX20 and PhX33 Pseudozyma hubeiensis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 native enzymes by anion exchange chromatography and gel filtration, PhX33 3.29fold, PhX20 5.73fold Pseudozyma hubeiensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 407
-
 purified PhX33 Pseudozyma hubeiensis
3.2.1.8 710
-
 purified PhX20 Pseudozyma hubeiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O
-
 Pseudozyma hubeiensis xylooligosaccharides
-
 ?
3.2.1.8 beta-1,4-xylan + H2O
-
 Pseudozyma hubeiensis NCIM 3574 xylooligosaccharides
-
 ?
3.2.1.8 beta-1,4-xylan + H2O PhX33 possesses three tryptophan and one carboxyl residues at the active site. The active site of PhX20 comprises one residue each of tryptophan, carboxyl and histidine Pseudozyma hubeiensis xylotriose + xylotetraose + xylopentaose + xylopentaose + xylohexaose + xyloheptaose both the xylanases PhX33 and PhX20 produce only xylooligosaccharides with degree of polymerization 3-7 without formation of xylose and xylobiose. PhX33 hydrolyzes xylan to xylotriose, xylotetraose and xylopentaose and PhX20 gives xylotriose, xylotetraose, xylopentaose, xylohexaose, and xyloheptaose ?
3.2.1.8 beta-1,4-xylan + H2O PhX33 possesses three tryptophan and one carboxyl residues at the active site. The active site of PhX20 comprises one residue each of tryptophan, carboxyl and histidine Pseudozyma hubeiensis NCIM 3574 xylotriose + xylotetraose + xylopentaose + xylopentaose + xylohexaose + xyloheptaose both the xylanases PhX33 and PhX20 produce only xylooligosaccharides with degree of polymerization 3-7 without formation of xylose and xylobiose. PhX33 hydrolyzes xylan to xylotriose, xylotetraose and xylopentaose and PhX20 gives xylotriose, xylotetraose, xylopentaose, xylohexaose, and xyloheptaose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 33500, about, PhX33, mass spectrometry, x * 20100, about, PhX20, mass spectrometry Pseudozyma hubeiensis
3.2.1.8 More PhX33 contains predominant alpha-and, while PhX22 contains predominantly beta-sheets Pseudozyma hubeiensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 PhX20
-
 Pseudozyma hubeiensis
3.2.1.8 PhX33
-
 Pseudozyma hubeiensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
 assay at Pseudozyma hubeiensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 55 60 PhX20 Pseudozyma hubeiensis
3.2.1.8 60 65 PhX33 Pseudozyma hubeiensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 60
-
 purified PhX20, half-life is 10 min Pseudozyma hubeiensis
3.2.1.8 70
-
 purified PhX33, half-life is 15 min Pseudozyma hubeiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 1383
-
 beta-1,4-xylan pH 7.0, 60°C, PhX33, oat spelt xylan Pseudozyma hubeiensis
3.2.1.8 3333
-
 beta-1,4-xylan pH 7.0, 60°C, PhX20, oat spelt xylan Pseudozyma hubeiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 4.2
-
 PhX20, isoelectric focusing Pseudozyma hubeiensis
3.2.1.8 4.7 6 PhX33, isoelectric focusing Pseudozyma hubeiensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 2 9 PhX20, 90% activity remains after 48 h Pseudozyma hubeiensis
3.2.1.8 3 9 PhX33, 90% activity remains after 48 h Pseudozyma hubeiensis