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Differential role of cathepsins B and L in autophagy-associated cell death induced by arsenic trioxide in U87 human glioblastoma cells

Pucer, A.; Castino, R.; Mirkovi?, B.; Falnoga, I.; Slejkovec, Z.; Isidoro, C.; Lah, T.T.; Biol. Chem. 391, 519-531 (2010)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.4.22.1
additional information
downregulation of CatB by siRNA leads to 33% residual activity of CatB, but has no effect on caspase 3/7 activation
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.22.1
arsenite
i.e. arsenic trioxide, inhibits CatB in glioblastoma cells, 20% inhibition at 0.022 mM, 50% at 0.020 mM
Homo sapiens
3.4.22.1
CA-074-Me
strong inhibition
Homo sapiens
3.4.22.1
CLIK148
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.22.1
lysosome
-
Homo sapiens
5764
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.22.1
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.22.1
glioblastoma cell
upregulation of CatB
Homo sapiens
-
3.4.22.1
U-87MG cell
upregulation of CatB
Homo sapiens
-
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.4.22.1
additional information
downregulation of CatB by siRNA leads to 33% residual activity of CatB, but has no effect on caspase 3/7 activation
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.22.1
arsenite
i.e. arsenic trioxide, inhibits CatB in glioblastoma cells, 20% inhibition at 0.022 mM, 50% at 0.020 mM
Homo sapiens
3.4.22.1
CA-074-Me
strong inhibition
Homo sapiens
3.4.22.1
CLIK148
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.22.1
lysosome
-
Homo sapiens
5764
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.22.1
glioblastoma cell
upregulation of CatB
Homo sapiens
-
3.4.22.1
U-87MG cell
upregulation of CatB
Homo sapiens
-
General Information
EC Number
General Information
Commentary
Organism
3.4.22.1
malfunction
arsenite treatment of human glioblastoma cells induces autophagosome formation and permeabilization of mitochondria, followed by caspase 3/7-mediated apoptosis. Arsenite toxicity involves a complex interplay between autophagy and apoptosis in human glioblastoma cells and is associated with inhibition of CatB, mechanism, overview
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.4.22.1
malfunction
arsenite treatment of human glioblastoma cells induces autophagosome formation and permeabilization of mitochondria, followed by caspase 3/7-mediated apoptosis. Arsenite toxicity involves a complex interplay between autophagy and apoptosis in human glioblastoma cells and is associated with inhibition of CatB, mechanism, overview
Homo sapiens