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Literature summary extracted from

  • Ozaki, T.; Yamashita, T.; Ishiguro, S.
    Mitochondrial m-calpain plays a role in the release of truncated apoptosis-inducing factor from the mitochondria (2009), Biochim. Biophys. Acta, 1793, 1848-1859.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.52 calpastatin
-
 Rattus norvegicus
3.4.22.52 PD150606
-
 Rattus norvegicus
3.4.22.53 EGTA
-
 Rattus norvegicus
3.4.22.53 PD150606
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.52 cytosol
-
 Rattus norvegicus 5829
-
3.4.22.53 cytosol
-
 Rattus norvegicus 5829
-
3.4.22.53 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.52 Ca2+ micromolar (0.005-0.05 mM) Ca2+ concentrations are required for activation in vitro Rattus norvegicus
3.4.22.53 Ca2+ the Ca2+ dependency of mitochondrial m-calpain is similar to that of cytosolic m-calpain, 1 mM Ca2+ activates. Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.52 30000
-
 1 * 80000 + 1 * 30000, SDS-PAGE Rattus norvegicus
3.4.22.52 80000
-
 1 * 80000 + 1 * 30000, SDS-PAGE Rattus norvegicus
3.4.22.53 79920
-
 m-calpain large subunit, calculated from amino acid sequence Rattus norvegicus
3.4.22.53 80000
-
 1 * 80000 + 1 * ?, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.52 apoptosis-inducing factor + H2O Rattus norvegicus
-
 truncated apoptosis-inducing factor + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.52 Rattus norvegicus
-
-
-
3.4.22.53 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.53 DEAE-Sepharose column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.52 erythrocyte
-
 Rattus norvegicus
-
3.4.22.52 liver
-
 Rattus norvegicus
-
3.4.22.53 erythrocyte
-
 Rattus norvegicus
-
3.4.22.53 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.52 apoptosis-inducing factor + H2O
-
 Rattus norvegicus truncated apoptosis-inducing factor + ?
-
 ?
3.4.22.53 casein + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.22.53 voltage-dependent anion channel + H2O mitochondrial m-calpain truncates voltage-dependent anion channel in Ca2+-dependent manner Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.52 heterodimer 1 * 80000 + 1 * 30000, SDS-PAGE Rattus norvegicus
3.4.22.53 heterodimer 1 * 80000 + 1 * ?, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.52 mu-calpain
-
 Rattus norvegicus
3.4.22.53 calpain 2
-
 Rattus norvegicus
3.4.22.53 mitochondrial m-calpain m-calpain large subunit Rattus norvegicus

General Information

EC Number General Information Comment Organism
3.4.22.53 physiological function mitochondrial m-calpain plays a role in the release of truncated apoptosis-inducing factor from the mitochondria by cleaving voltage-dependent anion channel Rattus norvegicus