BRENDA - Enzyme Database

The mechanisms of human hotdog-fold thioesterase 2 (hTHEM2) substrate recognition and catalysis illuminated by a structure and function based analysis

Cao, J.; Xu, H.; Zhao, H.; Gong, W.; Dunaway-Mariano, D.; Biochemistry 48, 1293-1304 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.2.20
His-tagged version expressed in Escherichia coli BL21(DE3)
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.1.2.20
hanging drop vapor diffusion method
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.2.20
D65A
active site residue
Homo sapiens
3.1.2.20
D65E
active site residue
Homo sapiens
3.1.2.20
D65N
active site residue
Homo sapiens
3.1.2.20
H134A
reduction in catalytic efficiency
Homo sapiens
3.1.2.20
H137A
conserved among vertebrate homologues
Homo sapiens
3.1.2.20
H56A
active site residue
Homo sapiens
3.1.2.20
K136A
relatively small perturbation in catalytic efficiency
Homo sapiens
3.1.2.20
N50A
active site residue
Homo sapiens
3.1.2.20
S83A
active site residue
Homo sapiens
3.1.2.20
T69A
active site residue
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.20
desulfoCoA
-
Homo sapiens
3.1.2.20
phenylacetylmethyl-CoA
-
Homo sapiens
3.1.2.20
undecan-2-one-CoA
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.20
0.0049
-
n-decanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.005
-
myristoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.009
-
myristoyl-CoA
wild-type protein, pH7.5, 37C
Homo sapiens
3.1.2.20
0.009
-
oleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.009
-
palmitoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.0099
-
lauroyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.01
-
3,4-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.016
-
palmitoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.02
-
Arachidonoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.02
-
stearoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.025
-
3,5-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.026
-
3-hydroxyphenylacetyl-CoA
H56A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.026
-
n-octanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.032
-
3-hydroxybenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.049
-
4-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.07
-
n-hexanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.11
-
beta-hydroxybutyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.12
-
3-hydroxyphenylacetyl-CoA
K136A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.12
-
beta-methylcrotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.13
-
4-chlorobenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.16
-
3-hydroxyphenylacetyl-CoA
T69A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.18
-
crotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.19
-
n-propionyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.21
-
methylmalonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.22
-
2-butenoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.23
-
3-hydroxyphenylacetyl-CoA
D65E mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.24
-
phenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.25
-
tiglyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.28
-
malonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.29
-
acetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.3
-
3-hydroxyphenylacetyl-CoA
N50A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.31
-
3-hydroxyphenylacetyl-CoA
S83A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.31
-
linoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.36
-
3-hydroxyphenylacetyl-CoA
D65N mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.41
-
3-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.6
-
n-butyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.62
-
3-hydroxyphenylacetyl-CoA
H134A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.67
-
glutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.9
-
3-hydroxyphenylacetyl-CoA
H137A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
2.9
-
3-hydroxy-3-methylglutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.20
Homo sapiens
Q9NPJ3
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.20
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.20
2-butenoyl-CoA + H2O
-
707486
Homo sapiens
CoA + 2-butenoate
-
-
-
?
3.1.2.20
3,4-dihydroxyphenylacetyl-CoA + H2O
-
707486
Homo sapiens
CoA + 3,4-dihydroxyphenylacetate
-
-
-
?
3.1.2.20
3,5-dihydroxyphenylacetyl-CoA + H2O
-
707486
Homo sapiens
CoA + 3,5-dihydroxyphenylacetate
-
-
-
?
3.1.2.20
3-hydroxy-3-methylglutaryl-CoA + H2O
-
707486
Homo sapiens
CoA + 3-hydroxy-3-methylglutarate
-
-
-
?
3.1.2.20
3-hydroxybenzoyl-CoA + H2O
-
707486
Homo sapiens
CoA + 3-hydroxybenzoate
-
-
-
?
3.1.2.20
3-hydroxyphenylacetyl-CoA + H2O
-
707486
Homo sapiens
CoA + 3-hydroxyphenylacetate
-
-
-
?
3.1.2.20
4-chlorobenzoyl-CoA + H2O
-
707486
Homo sapiens
CoA + 4-chlorobenzoate
-
-
-
?
3.1.2.20
4-hydroxyphenylacetyl-CoA + H2O
-
707486
Homo sapiens
CoA + 4-hydroxyphenylacetate
-
-
-
?
3.1.2.20
acetyl-CoA + H2O
-
707486
Homo sapiens
CoA + acetate
-
-
-
?
3.1.2.20
arachidonoyl-CoA + H2O
-
707486
Homo sapiens
CoA + arachidonate
-
-
-
?
3.1.2.20
beta-hydroxybutyryl-CoA + H2O
-
707486
Homo sapiens
CoA + beta-hydroxybutyrate
-
-
-
?
3.1.2.20
beta-methylcrotonyl-CoA + H2O
-
707486
Homo sapiens
CoA + beta-methylcrotonate
-
-
-
?
3.1.2.20
crotonyl-CoA + H2O
-
707486
Homo sapiens
CoA + crotonate
-
-
-
?
3.1.2.20
glutaryl-CoA + H2O
-
707486
Homo sapiens
CoA + glutarate
-
-
-
?
3.1.2.20
lauroyl-CoA + H2O
-
707486
Homo sapiens
CoA + laurate
-
-
-
?
3.1.2.20
linoleoyl-CoA + H2O
-
707486
Homo sapiens
CoA + linoleate
-
-
-
?
3.1.2.20
malonyl-CoA + H2O
-
707486
Homo sapiens
CoA + malonate
-
-
-
?
3.1.2.20
methylmalonyl-CoA + H2O
-
707486
Homo sapiens
CoA + methylmalonate
-
-
-
?
3.1.2.20
myristoyl-CoA + H2O
-
707486
Homo sapiens
CoA + myristate
-
-
-
?
3.1.2.20
n-butyryl-CoA + H2O
-
707486
Homo sapiens
CoA + n-butyrate
-
-
-
?
3.1.2.20
n-decanoyl-CoA + H2O
-
707486
Homo sapiens
CoA + n-decanoate
-
-
-
?
3.1.2.20
n-hexanoyl-CoA + H2O
-
707486
Homo sapiens
CoA + n-hexanoate
-
-
-
?
3.1.2.20
n-octanoyl-CoA + H2O
-
707486
Homo sapiens
CoA + n-octanoate
-
-
-
?
3.1.2.20
n-propionyl-CoA + H2O
-
707486
Homo sapiens
CoA + n-propionate
-
-
-
?
3.1.2.20
oleoyl-CoA + H2O
-
707486
Homo sapiens
CoA + oleate
-
-
-
?
3.1.2.20
palmitoleoyl-CoA + H2O
-
707486
Homo sapiens
CoA + palmitoleate
-
-
-
?
3.1.2.20
palmitoyl-CoA + H2O
-
707486
Homo sapiens
CoA + palmitate
-
-
-
?
3.1.2.20
phenylacetyl-CoA + H2O
-
707486
Homo sapiens
CoA + phenylacetate
-
-
-
?
3.1.2.20
stearoyl-CoA + H2O
-
707486
Homo sapiens
CoA + stearate
-
-
-
?
3.1.2.20
tiglyl-CoA + H2O
-
707486
Homo sapiens
CoA + (2E)-2-methylbut-2-enoate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.20
homotetramer
crystal structure
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.20
additional information
-
additional information
no activity observed with 4-hydroxybenzoyl-CoA, lauroyl-acyl carrier protein, myristoyl-acyl carrier protein, palmitoyl-acyl carrier protein, octanoyl-acyl carrier protein, oleoyl-acyl carrier protein and acylated peptide N-acetyl-Gly-Ala-Cys-S-palmitoyl, turnover number of mutant protein D65A below 0.00001 s-1 for 3-hydroxyphenylacetyl-CoA
Homo sapiens
3.1.2.20
0.0032
-
4-chlorobenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.0044
-
palmitoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.0047
-
n-octanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.007
-
3-hydroxyphenylacetyl-CoA
N50A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.0076
-
crotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.011
-
3-hydroxybenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.011
-
oleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.011
-
stearoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.014
-
2-butenoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.016
-
n-decanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.017
-
linoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.017
-
palmitoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.019
-
lauroyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.02
-
myristoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.02
-
tiglyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.022
-
Arachidonoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.022
-
n-hexanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.023
-
beta-methylcrotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.036
-
n-propionyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.047
-
n-butyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.05
-
glutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.054
-
acetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.066
-
malonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.07
-
myristoyl-CoA
wild-type protein, pH7.5, 37C
Homo sapiens
3.1.2.20
0.079
-
3-hydroxy-3-methylglutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.083
-
methylmalonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.084
-
phenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.14
-
4-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.15
-
3-hydroxyphenylacetyl-CoA
S83A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.16
-
3-hydroxyphenylacetyl-CoA
D65E mutant protein, pH7.5, 25C; H134A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.18
-
beta-hydroxybutyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.19
-
3,4-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.19
-
3,5-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.32
-
3-hydroxyphenylacetyl-CoA
K136A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.36
-
3-hydroxyphenylacetyl-CoA
D65N mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.62
-
3-hydroxyphenylacetyl-CoA
H56A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.66
-
3-hydroxyphenylacetyl-CoA
T69A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.83
-
3-hydroxyphenylacetyl-CoA
H137A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.4
-
3-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.2.20
0.003
-
undecan-2-one-CoA
0 mM NaCl
Homo sapiens
3.1.2.20
0.0033
-
undecan-2-one-CoA
100 mM NaCl
Homo sapiens
3.1.2.20
0.009
-
desulfoCoA
-
Homo sapiens
3.1.2.20
0.5
-
phenylacetylmethyl-CoA
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.20
His-tagged version expressed in Escherichia coli BL21(DE3)
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.1.2.20
hanging drop vapor diffusion method
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.2.20
D65A
active site residue
Homo sapiens
3.1.2.20
D65E
active site residue
Homo sapiens
3.1.2.20
D65N
active site residue
Homo sapiens
3.1.2.20
H134A
reduction in catalytic efficiency
Homo sapiens
3.1.2.20
H137A
conserved among vertebrate homologues
Homo sapiens
3.1.2.20
H56A
active site residue
Homo sapiens
3.1.2.20
K136A
relatively small perturbation in catalytic efficiency
Homo sapiens
3.1.2.20
N50A
active site residue
Homo sapiens
3.1.2.20
S83A
active site residue
Homo sapiens
3.1.2.20
T69A
active site residue
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.20
desulfoCoA
-
Homo sapiens
3.1.2.20
phenylacetylmethyl-CoA
-
Homo sapiens
3.1.2.20
undecan-2-one-CoA
-
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.2.20
0.003
-
undecan-2-one-CoA
0 mM NaCl
Homo sapiens
3.1.2.20
0.0033
-
undecan-2-one-CoA
100 mM NaCl
Homo sapiens
3.1.2.20
0.009
-
desulfoCoA
-
Homo sapiens
3.1.2.20
0.5
-
phenylacetylmethyl-CoA
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.20
0.0049
-
n-decanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.005
-
myristoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.009
-
myristoyl-CoA
wild-type protein, pH7.5, 37C
Homo sapiens
3.1.2.20
0.009
-
oleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.009
-
palmitoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.0099
-
lauroyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.01
-
3,4-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.016
-
palmitoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.02
-
Arachidonoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.02
-
stearoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.025
-
3,5-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.026
-
3-hydroxyphenylacetyl-CoA
H56A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.026
-
n-octanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.032
-
3-hydroxybenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.049
-
4-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.07
-
n-hexanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.11
-
beta-hydroxybutyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.12
-
3-hydroxyphenylacetyl-CoA
K136A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.12
-
beta-methylcrotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.13
-
4-chlorobenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.16
-
3-hydroxyphenylacetyl-CoA
T69A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.18
-
crotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.19
-
n-propionyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.21
-
methylmalonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.22
-
2-butenoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.23
-
3-hydroxyphenylacetyl-CoA
D65E mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.24
-
phenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.25
-
tiglyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.28
-
malonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.29
-
acetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.3
-
3-hydroxyphenylacetyl-CoA
N50A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.31
-
3-hydroxyphenylacetyl-CoA
S83A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.31
-
linoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.36
-
3-hydroxyphenylacetyl-CoA
D65N mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.41
-
3-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.6
-
n-butyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.62
-
3-hydroxyphenylacetyl-CoA
H134A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.67
-
glutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.9
-
3-hydroxyphenylacetyl-CoA
H137A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
2.9
-
3-hydroxy-3-methylglutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.20
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.20
2-butenoyl-CoA + H2O
-
707486
Homo sapiens
CoA + 2-butenoate
-
-
-
?
3.1.2.20
3,4-dihydroxyphenylacetyl-CoA + H2O
-
707486
Homo sapiens
CoA + 3,4-dihydroxyphenylacetate
-
-
-
?
3.1.2.20
3,5-dihydroxyphenylacetyl-CoA + H2O
-
707486
Homo sapiens
CoA + 3,5-dihydroxyphenylacetate
-
-
-
?
3.1.2.20
3-hydroxy-3-methylglutaryl-CoA + H2O
-
707486
Homo sapiens
CoA + 3-hydroxy-3-methylglutarate
-
-
-
?
3.1.2.20
3-hydroxybenzoyl-CoA + H2O
-
707486
Homo sapiens
CoA + 3-hydroxybenzoate
-
-
-
?
3.1.2.20
3-hydroxyphenylacetyl-CoA + H2O
-
707486
Homo sapiens
CoA + 3-hydroxyphenylacetate
-
-
-
?
3.1.2.20
4-chlorobenzoyl-CoA + H2O
-
707486
Homo sapiens
CoA + 4-chlorobenzoate
-
-
-
?
3.1.2.20
4-hydroxyphenylacetyl-CoA + H2O
-
707486
Homo sapiens
CoA + 4-hydroxyphenylacetate
-
-
-
?
3.1.2.20
acetyl-CoA + H2O
-
707486
Homo sapiens
CoA + acetate
-
-
-
?
3.1.2.20
arachidonoyl-CoA + H2O
-
707486
Homo sapiens
CoA + arachidonate
-
-
-
?
3.1.2.20
beta-hydroxybutyryl-CoA + H2O
-
707486
Homo sapiens
CoA + beta-hydroxybutyrate
-
-
-
?
3.1.2.20
beta-methylcrotonyl-CoA + H2O
-
707486
Homo sapiens
CoA + beta-methylcrotonate
-
-
-
?
3.1.2.20
crotonyl-CoA + H2O
-
707486
Homo sapiens
CoA + crotonate
-
-
-
?
3.1.2.20
glutaryl-CoA + H2O
-
707486
Homo sapiens
CoA + glutarate
-
-
-
?
3.1.2.20
lauroyl-CoA + H2O
-
707486
Homo sapiens
CoA + laurate
-
-
-
?
3.1.2.20
linoleoyl-CoA + H2O
-
707486
Homo sapiens
CoA + linoleate
-
-
-
?
3.1.2.20
malonyl-CoA + H2O
-
707486
Homo sapiens
CoA + malonate
-
-
-
?
3.1.2.20
methylmalonyl-CoA + H2O
-
707486
Homo sapiens
CoA + methylmalonate
-
-
-
?
3.1.2.20
myristoyl-CoA + H2O
-
707486
Homo sapiens
CoA + myristate
-
-
-
?
3.1.2.20
n-butyryl-CoA + H2O
-
707486
Homo sapiens
CoA + n-butyrate
-
-
-
?
3.1.2.20
n-decanoyl-CoA + H2O
-
707486
Homo sapiens
CoA + n-decanoate
-
-
-
?
3.1.2.20
n-hexanoyl-CoA + H2O
-
707486
Homo sapiens
CoA + n-hexanoate
-
-
-
?
3.1.2.20
n-octanoyl-CoA + H2O
-
707486
Homo sapiens
CoA + n-octanoate
-
-
-
?
3.1.2.20
n-propionyl-CoA + H2O
-
707486
Homo sapiens
CoA + n-propionate
-
-
-
?
3.1.2.20
oleoyl-CoA + H2O
-
707486
Homo sapiens
CoA + oleate
-
-
-
?
3.1.2.20
palmitoleoyl-CoA + H2O
-
707486
Homo sapiens
CoA + palmitoleate
-
-
-
?
3.1.2.20
palmitoyl-CoA + H2O
-
707486
Homo sapiens
CoA + palmitate
-
-
-
?
3.1.2.20
phenylacetyl-CoA + H2O
-
707486
Homo sapiens
CoA + phenylacetate
-
-
-
?
3.1.2.20
stearoyl-CoA + H2O
-
707486
Homo sapiens
CoA + stearate
-
-
-
?
3.1.2.20
tiglyl-CoA + H2O
-
707486
Homo sapiens
CoA + (2E)-2-methylbut-2-enoate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.20
homotetramer
crystal structure
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.20
additional information
-
additional information
no activity observed with 4-hydroxybenzoyl-CoA, lauroyl-acyl carrier protein, myristoyl-acyl carrier protein, palmitoyl-acyl carrier protein, octanoyl-acyl carrier protein, oleoyl-acyl carrier protein and acylated peptide N-acetyl-Gly-Ala-Cys-S-palmitoyl, turnover number of mutant protein D65A below 0.00001 s-1 for 3-hydroxyphenylacetyl-CoA
Homo sapiens
3.1.2.20
0.0032
-
4-chlorobenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.0044
-
palmitoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.0047
-
n-octanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.007
-
3-hydroxyphenylacetyl-CoA
N50A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.0076
-
crotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.011
-
3-hydroxybenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.011
-
oleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.011
-
stearoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.014
-
2-butenoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.016
-
n-decanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.017
-
linoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.017
-
palmitoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.019
-
lauroyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.02
-
myristoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.02
-
tiglyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.022
-
Arachidonoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.022
-
n-hexanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.023
-
beta-methylcrotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.036
-
n-propionyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.047
-
n-butyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.05
-
glutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.054
-
acetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.066
-
malonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.07
-
myristoyl-CoA
wild-type protein, pH7.5, 37C
Homo sapiens
3.1.2.20
0.079
-
3-hydroxy-3-methylglutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.083
-
methylmalonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.084
-
phenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.14
-
4-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.15
-
3-hydroxyphenylacetyl-CoA
S83A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.16
-
3-hydroxyphenylacetyl-CoA
D65E mutant protein, pH7.5, 25C; H134A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.18
-
beta-hydroxybutyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.19
-
3,4-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.19
-
3,5-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.32
-
3-hydroxyphenylacetyl-CoA
K136A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.36
-
3-hydroxyphenylacetyl-CoA
D65N mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.62
-
3-hydroxyphenylacetyl-CoA
H56A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.66
-
3-hydroxyphenylacetyl-CoA
T69A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.83
-
3-hydroxyphenylacetyl-CoA
H137A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.4
-
3-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.1.2.20
0.023
-
3-hydroxyphenylacetyl-CoA
N50A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.025
-
4-chlorobenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.027
-
3-hydroxy-3-methylglutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.054
-
linoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.064
-
2-butenoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.078
-
n-butyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.082
-
tiglyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.17
-
3-hydroxyphenylacetyl-CoA
H134A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.18
-
acetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.18
-
n-octanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.19
-
beta-methylcrotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.19
-
n-propionyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.24
-
malonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.28
-
palmitoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.31
-
n-hexanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.35
-
3-hydroxybenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.35
-
phenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.39
-
methylmalonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.47
-
crotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.58
-
3-hydroxyphenylacetyl-CoA
S83A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.67
-
3-hydroxyphenylacetyl-CoA
D65E mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.75
-
glutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.92
-
3-hydroxyphenylacetyl-CoA
H137A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1
-
3-hydroxyphenylacetyl-CoA
D65N mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.1
-
Arachidonoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.3
-
oleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.6
-
beta-hydroxybutyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.9
-
lauroyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.9
-
palmitoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
2.6
-
3-hydroxyphenylacetyl-CoA
K136A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
2.9
-
4-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
3.3
-
n-decanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
4
-
myristoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
4.1
-
3-hydroxyphenylacetyl-CoA
T69A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
5.5
-
stearoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
7.7
-
myristoyl-CoA
wild-type protein, pH7.5, 37C
Homo sapiens
3.1.2.20
7.9
-
3,5-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
18
-
3,4-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
24
-
3-hydroxyphenylacetyl-CoA
H56A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
35
-
3-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.1.2.20
0.023
-
3-hydroxyphenylacetyl-CoA
N50A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.025
-
4-chlorobenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.027
-
3-hydroxy-3-methylglutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.054
-
linoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.064
-
2-butenoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.078
-
n-butyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.082
-
tiglyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.17
-
3-hydroxyphenylacetyl-CoA
H134A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.18
-
acetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.18
-
n-octanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.19
-
beta-methylcrotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.19
-
n-propionyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.24
-
malonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.28
-
palmitoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.31
-
n-hexanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.35
-
3-hydroxybenzoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.35
-
phenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.39
-
methylmalonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.47
-
crotonyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.58
-
3-hydroxyphenylacetyl-CoA
S83A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.67
-
3-hydroxyphenylacetyl-CoA
D65E mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.75
-
glutaryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
0.92
-
3-hydroxyphenylacetyl-CoA
H137A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1
-
3-hydroxyphenylacetyl-CoA
D65N mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.1
-
Arachidonoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.3
-
oleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.6
-
beta-hydroxybutyryl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.9
-
lauroyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
1.9
-
palmitoleoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
2.6
-
3-hydroxyphenylacetyl-CoA
K136A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
2.9
-
4-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
3.3
-
n-decanoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
4
-
myristoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
4.1
-
3-hydroxyphenylacetyl-CoA
T69A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
5.5
-
stearoyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
7.7
-
myristoyl-CoA
wild-type protein, pH7.5, 37C
Homo sapiens
3.1.2.20
7.9
-
3,5-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
18
-
3,4-dihydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens
3.1.2.20
24
-
3-hydroxyphenylacetyl-CoA
H56A mutant protein, pH7.5, 25C
Homo sapiens
3.1.2.20
35
-
3-hydroxyphenylacetyl-CoA
wild-type protein, pH7.5, 25C
Homo sapiens