Literature summary extracted from

Domingues, S.; Matos, R.G.; Reis, F.P.; Fialho, A.M.; Barbas, A.; Arraiano, C.M.
Biochemical characterization of the RNase II family of exoribonucleases from the human pathogens Salmonella typhimurium and Streptococcus pneumoniae (2009), Biochemistry, 48, 11848-11857.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.13.1	into the pET-15b vector, cloned into Escherichia coli DH5alpha, subsequently transformed into Escherichia coli strain BL21(DE3)	Salmonella enterica subsp. enterica serovar Typhimurium
3.1.13.1	into the pET-15b vector, transformed into Escherichia coli Novablue, subsequently transformed into Escherichia coli strain BL21(DE3)	Streptococcus pneumoniae

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.13.1	Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-
3.1.13.1	Streptococcus pneumoniae	C6GKN7	strains T4 and TIGR4	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.13.1	by histidine affinity chromatography and an AKTA HPLC system	Salmonella enterica subsp. enterica serovar Typhimurium
3.1.13.1	by histidine affinity chromatography and an AKTA HPLC system	Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.13.1	poly(A) + H2O	35-nucleotide poly(A) chain, RNase II final end product contains four nucleotides, while RNase R renders a two-nucleotide fragment as the final product	Salmonella enterica subsp. enterica serovar Typhimurium	5'-AMP + oligonucleotide	-	?
3.1.13.1	poly(A) + H2O	35-nucleotide poly(A) chain, RNase R renders a two-nucleotide fragment as the final product	Streptococcus pneumoniae	5'-AMP + oligonucleotide	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.13.1	RNase II	-	Salmonella enterica subsp. enterica serovar Typhimurium
3.1.13.1	RNase R	-	Streptococcus pneumoniae
3.1.13.1	RNaseR	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)