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Literature summary extracted from

  • Willis, M.A.; Zhuang, Z.; Song, F.; Howard, A.; Dunaway-Mariano, D.; Herzberg, O.
    Structure of YciA from Haemophilus influenzae (HI0827), a hexameric broad specificity acyl-coenzyme A thioesterase (2008), Biochemistry, 47, 2797-2805.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.20 expressed in Escherichia coli BL21(DE3) Haemophilus influenzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.20 hanging drop vapor diffusion method Haemophilus influenzae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.20 D44A putative catalytic residue Haemophilus influenzae
3.1.2.20 N29A involved in substrate binding Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.20 0.0042
-
 lauroyl-CoA N29A mutant protein Haemophilus influenzae
3.1.2.20 0.0066
-
 lauroyl-CoA wild-type protein Haemophilus influenzae
3.1.2.20 0.013
-
 isobutyryl-CoA wild-type protein Haemophilus influenzae
3.1.2.20 0.092
-
 isobutyryl-CoA N29A mutant protein Haemophilus influenzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.20 17000
-
 6 * 17000, crystal structure analysis Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.20 Haemophilus influenzae P44886 stain Rd KW20
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.20
-
 Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.20 butyryl-CoA + H2O
-
 Haemophilus influenzae butanoate + CoA
-
 ?
3.1.2.20 butyrylpanthetheine phosphate + H2O
-
 Haemophilus influenzae pantetheine phosphate + butyrate
-
 ?
3.1.2.20 isobutyryl-CoA + H2O
-
 Haemophilus influenzae CoA + isobutyrate
-
 ?
3.1.2.20 lauroyl-CoA + H2O
-
 Haemophilus influenzae CoA + lauroate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.20 homohexamer 6 * 17000, crystal structure analysis Haemophilus influenzae

Synonyms

EC Number Synonyms Comment Organism
3.1.2.20 acyl-Coenzyme A thioesterase
-
 Haemophilus influenzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.20 additional information
-
 additional information D44A mutant protein: value below 0.001 s-1 for isobutyryl-CoA and lauroyl-CoA Haemophilus influenzae
3.1.2.20 0.08
-
 butyrylpantetheine phosphate wild-type protein Haemophilus influenzae
3.1.2.20 2.34
-
 isobutyryl-CoA N29A mutant protein Haemophilus influenzae
3.1.2.20 2.57
-
 lauroyl-CoA N29A mutant protein Haemophilus influenzae
3.1.2.20 6.7
-
 butyryl-CoA wild-type protein Haemophilus influenzae
3.1.2.20 9.2
-
 lauroyl-CoA wild-type protein Haemophilus influenzae
3.1.2.20 138
-
 isobutyryl-CoA wild-type protein Haemophilus influenzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.2.20 0.1
-
 butyrylpantetheine phosphate wild-type protein Haemophilus influenzae
3.1.2.20 25
-
 isobutyryl-CoA N29A mutant protein Haemophilus influenzae
3.1.2.20 100
-
 butyryl-CoA wild-type protein Haemophilus influenzae
3.1.2.20 610
-
 lauroyl-CoA N29A mutant protein Haemophilus influenzae
3.1.2.20 1400
-
 lauroyl-CoA wild-type protein Haemophilus influenzae
3.1.2.20 11000
-
 isobutyryl-CoA wild-type protein Haemophilus influenzae