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Literature summary extracted from

  • Grin, I.R.; Konorovsky, P.G.; Nevinsky, G.A.; Zharkov, D.O.
    Heavy metal ions affect the activity of DNA glycosylases of the fpg family (2009), Biochemistry (Moscow), 74, 1253-1259.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.23 Al3+
-
 Homo sapiens
3.2.2.23 Cd2+ preferentially binds to DNA bases rather than phosphates, the presence of the metal ions causes the enzyme to lose the ability for preferential binding to damaged DNA Homo sapiens
3.2.2.23 Co2+
-
 Homo sapiens
3.2.2.23 Cu2+ preferentially binds to DNA bases rather than phosphates, the presence of the metal ions causes the enzyme to lose the ability for preferential binding to damaged DNA Homo sapiens
3.2.2.23 Fe2+
-
 Homo sapiens
3.2.2.23 additional information differing inhibitory effects of metal ions in potassiumphosphate buffer and Tris-HCl buffer, mechanism of NEIL1 inhibition by heavy metal salts, e.g. involving sequential elimination of phosphate groups from the 3'- and 5'-side of the formed AP site, overview Homo sapiens
3.2.2.23 Ni2+
-
 Homo sapiens
3.2.2.23 Zn2+
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.23 additional information
-
 additional information steady state kinetics Homo sapiens
3.2.2.23 0.00015
-
 5,6-dihydrouracil-DNA NEIL1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.23 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.23 additional information NEIL1 is not affected by Mg2+, Mn2+, and Ca2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.23 5,6-dihydrouracil-DNA + H2O Homo sapiens DHU is excised from DNA by a number of DNA glycosylases including Fpg and Nei 5,6-dihydrouracil + DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.23 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.23 5,6-dihydrouracil-DNA + H2O DHU is excised from DNA by a number of DNA glycosylases including Fpg and Nei Homo sapiens 5,6-dihydrouracil + DNA
-
 ?
3.2.2.23 5,6-dihydrouracil-DNA + H2O DHU is formed in DNA from cytosine under the action of OH radicals under ionizing radiation Homo sapiens 5,6-dihydrouracil + DNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.23 formamidopyrimidineDNA glycosylase
-
 Homo sapiens
3.2.2.23 FPG
-
 Homo sapiens
3.2.2.23 More the enzymes belong to the Fpg family of DNA glycosylases Homo sapiens
3.2.2.23 Nei
-
 Homo sapiens
3.2.2.23 NEIL1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.23 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.23 0.02
-
 5,6-dihydrouracil-DNA NEIL1 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.23 7.5
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.2.23 0.007
-
-
 Homo sapiens Cd2+
3.2.2.23 0.016
-
-
 Homo sapiens Zn2+
3.2.2.23 0.4
-
-
 Homo sapiens Cu2+

General Information

EC Number General Information Comment Organism
3.2.2.23 physiological function the enzyme initiates the process of repair of oxidized DNA bases Homo sapiens