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Literature summary extracted from

  • Khairullin, R.F.; Mikhailova, A.G.; Sebyakina, T.Y.; Lubenets, N.L.; Ziganshin, R.H.; Demidyuk, I.V.; Gromova, T.Y.; Kostrov, S.V.; Rumsh, L.D.
    Oligopeptidase B from Serratia proteamaculans. I. Determination of primary structure, isolation, and purification of wild-type and recombinant enzyme variants (2009), Biochemistry (Moscow), 74, 1164-1172.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.83 expressed in Escherichia coli BL-21(DE3) cells Serratia proteamaculans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 methanol incubation in 20% (v/v) methanol (10-20 h, 4°C) results in partial decomposition of the high molecular weight PSP-GroEL complex with precipitation of denatured chaperonin and a small (no more than 10%) loss of PSP activity Serratia proteamaculans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.83 78000
-
 His-tagged enzyme, SDS-PAGE Serratia proteamaculans

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Serratia proteamaculans B3VI58
-
-
3.4.21.83 Serratia proteamaculans 94 B3VI58
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.83 QSepharose column chromatography, ultrafiltration, Ni-NTA-agarose column chromatography, and immobilized basic pancreatic trypsin inhibitor-Sepharose column chromatography Serratia proteamaculans

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
-
 Serratia proteamaculans Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?
3.4.21.83 Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
-
 Serratia proteamaculans 94 Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.83 oligopeptidase B
-
 Serratia proteamaculans
3.4.21.83 OpdB
-
 Serratia proteamaculans
3.4.21.83 PSP
-
 Serratia proteamaculans
3.4.21.83 trypsin-like protease
-
 Serratia proteamaculans