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Literature summary extracted from

  • Li, L.; Woodward, R.; Ding, Y.; Liu, X.W.; Yi, W.; Bhatt, V.S.; Chen, M.; Zhang, L.W.; Wang, P.G.
    Overexpression and topology of bacterial oligosaccharyltransferase PglB (2010), Biochem. Biophys. Res. Commun., 394, 1069-1074.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.99.18 PglB is overexpressed in Escherichia coli C43(DE3) at a level of 1 mg/L cell cultures Campylobacter jejuni
2.4.99.19 gene pglB, large-scale overexpression of His-tagged PglB in Escherichia coli strain C43(DE3) Campylobacter jejuni

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.99.18 membrane PglB possesses 11 transmembrane segments and two relatively large periplasmic regions other than the C-terminal domain Campylobacter jejuni 16020
-
2.4.99.19 membrane topological model of PglB with periplasmic, transmembrane, and cytoplasmic parts, overview Campylobacter jejuni 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.99.19 Mn2+ activates Campylobacter jejuni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.99.19 82000
-
 x * 82000, SDS-PAGE Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.99.19 tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine Campylobacter jejuni
-
 tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.18 Campylobacter jejuni Q9S4V7
-
-
2.4.99.19 Campylobacter jejuni
-
 gene pglB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.99.19 recombinant His-tagged PglB from Escherichia coli strain C43(DE3) by nickel affinity chromatography Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.18 pentaprenyl diphospho-N-acetyl-D-galactosamine + [protein]-L-asparagine PglB can utilize shorter polyisoprenol (pentaprenol) as the lipid carrier, albeit with reduced efficiency Campylobacter jejuni pentaprenyl diphosphate + [protein]-N-(N-acetyl-D-galactosaminyl)-L-asparagine
-
 ?
2.4.99.18 undecaprenyl diphospho-N-acetyl-D-galactosamine + [protein]-L-asparagine PglB is solely responsible for the oligosaccharyltransferase activity. PglB can transfer a monosaccharide, e.g. GalNAc to a peptide acceptor. PglB exhibits relaxed sugar substrate specificity Campylobacter jejuni undecaprenyl diphosphate + [protein]-N-(N-acetyl-D-galactosaminyl)-L-asparagine
-
 ?
2.4.99.19 Lys-Asp-Phe-Asn-Val-Ser-Lys-Ala + N-acetyl-alpha-D-galactosaminyl-diphospho-tritrans,heptacis-undecaprenol a chemically synthesized sugar donor with synthesized peptide acceptor Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + Lys-Asp-Phe-N4-[N-acetyl-beta-D-galactosaminyl]-Asn-Val-Ser-Lys-Ala
-
 ?
2.4.99.19 additional information PglB is solely responsible for the oligosaccharyltransferase activity, PglB exhibits relaxed sugar substrate specificity Campylobacter jejuni ?
-
 ?
2.4.99.19 tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine
-
 Campylobacter jejuni tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine ?

Subunits

EC Number Subunits Comment Organism
2.4.99.19 ? x * 82000, SDS-PAGE Campylobacter jejuni
2.4.99.19 More PglB is a protein with two domains, an N-terminal transmembrane domain with 9-13 predicted transmembrane segments, and a C-terminal soluble domain which contains the catalytic center Campylobacter jejuni

Synonyms

EC Number Synonyms Comment Organism
2.4.99.18 PglB
-
 Campylobacter jejuni
2.4.99.19 bacterial oligosaccharyltransferase
-
 Campylobacter jejuni
2.4.99.19 PglB
-
 Campylobacter jejuni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.99.19 30
-
 assay at Campylobacter jejuni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.99.18 7.5
-
 assay at Campylobacter jejuni
2.4.99.19 7.5
-
 assay at Campylobacter jejuni

General Information

EC Number General Information Comment Organism
2.4.99.19 additional information topology mapping of PglB using the PhoA/LacZ fusion method, overview. The topological model shows that PglB possesses 11 transmembrane segments and two relatively large periplasmic regions other than the C-terminal domain, which is consistent with the proposal of the common Ncyt-Cperi topology with 11 transmembrane segments for the STT3 family proteins Campylobacter jejuni