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Literature summary extracted from

  • Mander, P.; Simkhada, J.R.; Cho, S.S.; Park, S.J.; Choi, H.S.; Lee, H.C.; Sohng, J.K.; Yoo, J.C.
    A novel Ca2+-dependent phospholipase D from Streptomyces tendae, possessing only hydrolytic activity (2009), Arch. Pharm. Res., 32, 1461-1467.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 CHAPS activates 1.8fold at 1.5% Streptomyces tendae
3.1.4.4 deoxycholic acid activates 2.2fold at 1.5% Streptomyces tendae
3.1.4.4 polyethylene-4-laurylether activates 2.1fold at 1.5% Streptomyces tendae
3.1.4.4 Triton X-100 activates 2.65fold at 1.5% Streptomyces tendae
3.1.4.4 Tween 80 activates 2fold at 1.5% Streptomyces tendae
3.1.4.4 Tween-20 activates 2.3fold at 1.5% Streptomyces tendae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 EDTA complete inhibition at 2 mM Streptomyces tendae
3.1.4.4 N-laurylsarcosine 75% inhibition at 1.5% Streptomyces tendae
3.1.4.4 SDS complete inhibition at 1.5% Streptomyces tendae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 0.95
-
 phosphatidylcholine pH 8.0, 60°C Streptomyces tendae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.4 extracellular
-
 Streptomyces tendae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ absolutely required, best at 2 mM, cannot be substituted by other metal ions, overview Streptomyces tendae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 43000
-
 x * 43000, SDS-PAGE Streptomyces tendae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phosphatidylcholine + H2O Streptomyces tendae
-
 choline + phosphatidic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Streptomyces tendae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 native enzyme 4fold from culture supernatant by ammonium sulfate fractionation, dialysis, ultrafiltration, gel filtration and anion exchange chromatography Streptomyces tendae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 additional information
-
 the purified native enzyme shows a specific activity of 18 U/ml Streptomyces tendae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 additional information PLDSt cannot catalyze transphosphatidylation of glycerol, L-serine, myo-inositol and ethanolamine, the Streptomyces tendae PLD possesses only hydrolytic activity Streptomyces tendae ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Streptomyces tendae choline + phosphatidic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.4 ? x * 43000, SDS-PAGE Streptomyces tendae

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 Ca2+-dependent phospholipase D
-
 Streptomyces tendae
3.1.4.4 phosphatidylcholine choline hydrolase
-
 Streptomyces tendae
3.1.4.4 PLD
-
 Streptomyces tendae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 60
-
 hydrolase activity Streptomyces tendae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.4 40 70 purified enzyme, 1.5 h, over 90% of maximal activity Streptomyces tendae
3.1.4.4 65
-
 90 min, purified enzyme, stable Streptomyces tendae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 8
-
 hydrolase activity Streptomyces tendae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.4 6 11 activity range, profile, overview Streptomyces tendae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.4 7.5 11 purified enzyme, 24 h, over 80% of maximal activity within that range, over 95% at pH 8.0-10.0 Streptomyces tendae