EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.4 | CHAPS | activates 1.8fold at 1.5% | Streptomyces tendae | |
3.1.4.4 | deoxycholic acid | activates 2.2fold at 1.5% | Streptomyces tendae | |
3.1.4.4 | polyethylene-4-laurylether | activates 2.1fold at 1.5% | Streptomyces tendae | |
3.1.4.4 | Triton X-100 | activates 2.65fold at 1.5% | Streptomyces tendae | |
3.1.4.4 | Tween 80 | activates 2fold at 1.5% | Streptomyces tendae | |
3.1.4.4 | Tween-20 | activates 2.3fold at 1.5% | Streptomyces tendae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.4 | EDTA | complete inhibition at 2 mM | Streptomyces tendae | |
3.1.4.4 | N-laurylsarcosine | 75% inhibition at 1.5% | Streptomyces tendae | |
3.1.4.4 | SDS | complete inhibition at 1.5% | Streptomyces tendae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.4 | 0.95 | - |
phosphatidylcholine | pH 8.0, 60°C | Streptomyces tendae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.4 | extracellular | - |
Streptomyces tendae | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.4 | Ca2+ | absolutely required, best at 2 mM, cannot be substituted by other metal ions, overview | Streptomyces tendae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.4 | 43000 | - |
x * 43000, SDS-PAGE | Streptomyces tendae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.4 | phosphatidylcholine + H2O | Streptomyces tendae | - |
choline + phosphatidic acid | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.4 | Streptomyces tendae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.4 | native enzyme 4fold from culture supernatant by ammonium sulfate fractionation, dialysis, ultrafiltration, gel filtration and anion exchange chromatography | Streptomyces tendae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.4 | additional information | - |
the purified native enzyme shows a specific activity of 18 U/ml | Streptomyces tendae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.4 | additional information | PLDSt cannot catalyze transphosphatidylation of glycerol, L-serine, myo-inositol and ethanolamine, the Streptomyces tendae PLD possesses only hydrolytic activity | Streptomyces tendae | ? | - |
? | |
3.1.4.4 | phosphatidylcholine + H2O | - |
Streptomyces tendae | choline + phosphatidic acid | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.4 | ? | x * 43000, SDS-PAGE | Streptomyces tendae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.4 | Ca2+-dependent phospholipase D | - |
Streptomyces tendae |
3.1.4.4 | phosphatidylcholine choline hydrolase | - |
Streptomyces tendae |
3.1.4.4 | PLD | - |
Streptomyces tendae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.4 | 60 | - |
hydrolase activity | Streptomyces tendae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.4 | 40 | 70 | purified enzyme, 1.5 h, over 90% of maximal activity | Streptomyces tendae |
3.1.4.4 | 65 | - |
90 min, purified enzyme, stable | Streptomyces tendae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.4 | 8 | - |
hydrolase activity | Streptomyces tendae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.4 | 6 | 11 | activity range, profile, overview | Streptomyces tendae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.4 | 7.5 | 11 | purified enzyme, 24 h, over 80% of maximal activity within that range, over 95% at pH 8.0-10.0 | Streptomyces tendae |