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Literature summary extracted from

  • Peng, Y.; Chi, Z.M.; Wang, X.H.; Li, J.
    Purification and molecular characterization of exo-beta-1,3-glucanases from the marine yeast Williopsis saturnus WC91-2 (2009), Appl. Microbiol. Biotechnol., 85, 85-94.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 1,10-phenanthroline
-
 Cyberlindnera saturnus
3.2.1.58 Ag+
-
 Cyberlindnera saturnus
3.2.1.58 Ca2+
-
 Cyberlindnera saturnus
3.2.1.58 Co2+
-
 Cyberlindnera saturnus
3.2.1.58 Cu2+
-
 Cyberlindnera saturnus
3.2.1.58 EDTA
-
 Cyberlindnera saturnus
3.2.1.58 EGTA
-
 Cyberlindnera saturnus
3.2.1.58 Fe2+
-
 Cyberlindnera saturnus
3.2.1.58 Fe3+
-
 Cyberlindnera saturnus
3.2.1.58 Hg2+
-
 Cyberlindnera saturnus
3.2.1.58 iodoacetic acid
-
 Cyberlindnera saturnus
3.2.1.58 K+
-
 Cyberlindnera saturnus
3.2.1.58 Mg2+
-
 Cyberlindnera saturnus
3.2.1.58 Mn2+
-
 Cyberlindnera saturnus
3.2.1.58 Na+
-
 Cyberlindnera saturnus
3.2.1.58 PMSF
-
 Cyberlindnera saturnus
3.2.1.58 Zn2+
-
 Cyberlindnera saturnus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.58 soluble
-
 Cyberlindnera saturnus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.58 Ba2+ activates Cyberlindnera saturnus
3.2.1.58 Li+ activates Cyberlindnera saturnus
3.2.1.58 Ni2+ activates Cyberlindnera saturnus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.58 47500
-
 x * 47500, native enzyme, SDS-PAGE Cyberlindnera saturnus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.58 laminarin + H2O Cyberlindnera saturnus exo-beta-1,3-glucanases hydrolyze laminarin by sequentially cleaving glucose residues from the non-reducing end of polymers or oligomers ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Cyberlindnera saturnus C3VMY4 i.e. Hansenula saturnus, a marine yeast, strain WC91-2, gene WsEXG1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.58 soluble native enzyme 115fold from strain WC91-2 to homogeneity by ultrafiltration, gel filtration, and anion exchange chromatography Cyberlindnera saturnus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.58 1966
-
 purified native enzyme Cyberlindnera saturnus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 laminarin + H2O exo-beta-1,3-glucanases hydrolyze laminarin by sequentially cleaving glucose residues from the non-reducing end of polymers or oligomers Cyberlindnera saturnus ?
-
 ?
3.2.1.58 laminarin + H2O the purified native enzyme can convert laminarin into monosaccharides and disaccharides, but has no killer toxin activity Cyberlindnera saturnus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 47500, native enzyme, SDS-PAGE Cyberlindnera saturnus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.58 exo-beta-1,3-glucanase
-
 Cyberlindnera saturnus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 40
-
-
 Cyberlindnera saturnus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.58 20 60 activity range Cyberlindnera saturnus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 4
-
-
 Cyberlindnera saturnus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.58 3 7 activity range Cyberlindnera saturnus