EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.8.1 | acetaminophen | at 37°C, 5% slightly reduces activity by 18% concentration | Homo sapiens | |
3.1.8.1 | acetone | at 37°C, 1 mM reduces activity by 82% | Homo sapiens | |
3.1.8.1 | acetyl salicylic acid | at 37°C, 5% slightly reduces activity by 10% concentration | Homo sapiens | |
3.1.8.1 | beta-mercaptoethanol | at 37°C, 1 mM reduces activity by 52% | Homo sapiens | |
3.1.8.1 | Cd2+ | at 37°C, 1 mM inhibits activity | Homo sapiens | |
3.1.8.1 | Co2+ | at 37°C, 1 mM reduces activity by 30% | Homo sapiens | |
3.1.8.1 | Cu2+ | at 37°C, 1 mM inhibits activity | Homo sapiens | |
3.1.8.1 | dimethyl sulfoxide | at 37°C, 5% completely inhibits activity | Homo sapiens | |
3.1.8.1 | dimethylformamide | at 37°C, 5% completely inhibits activity | Homo sapiens | |
3.1.8.1 | ethanol | at 37°C, 1 mM reduces activity by 43% | Homo sapiens | |
3.1.8.1 | gentamicin sulfate | at 37°C, 0.6 mg/ml reduces activity by 50% | Homo sapiens | |
3.1.8.1 | iodoacetic acid | at 37°C, 5% completely inhibits activity | Homo sapiens | |
3.1.8.1 | kanamycin sulfate | at 37°C, 0.6 mg/ml reduces activity by 50% | Homo sapiens | |
3.1.8.1 | additional information | at 37°C, 1 mM Mg2+, 1 mM Mn2+, 5% glycerol, 5% PEG 6000, 5% ammonium sulfate, and 5% phenylmethylsulfonyl fluoride do not inhibit the enzyme activity | Homo sapiens | |
3.1.8.1 | Ni2+ | at 37°C, 1 mM inhibits activity | Homo sapiens | |
3.1.8.1 | Pb2+ | at 37°C, 1 mM reduces activity by 35% | Homo sapiens | |
3.1.8.1 | sodium ampicillin | at 37°C, 30 mg/ml reduces activity by 50% | Homo sapiens | |
3.1.8.1 | Sodium dodecyl sulfate | at 37°C, 5% completely inhibits activity | Homo sapiens | |
3.1.8.1 | streptomycin sulfate | at 37°C, 0.6 mg/ml reduces activity by 50% | Homo sapiens | |
3.1.8.1 | Zn2+ | at 37°C, 1 mM reduces activity by 70% | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.2 | 0.74 | - |
phenyl acetate | 2 mM substrate, at pH 8.0 and 37°C | Homo sapiens | |
3.1.8.1 | 1.3 | - |
paraoxon | 2 mM substrate, at pH 8.0 and 37°C | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.8.1 | additional information | at 37°C, 1 mM Mg2+ and Mn2+ have no stimulatory effect on activity | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.2 | 45000 | - |
x * 45000, SDS-PAGE | Homo sapiens |
3.1.8.1 | 45000 | - |
x * 45000, SDS-PAGE | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.2 | Homo sapiens | - |
- |
- |
3.1.8.1 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.2 | by Triton-X-100-treatment, ammonium sulfate precipitation, cholesterol-conjugated magnetic nanoparticles and gel filtration, at 4°C, to homogeneity, 515fold with 73% yield | Homo sapiens |
3.1.8.1 | by Triton-X-100-treatment, ammonium sulfate precipitation, cholesterol-conjugated magnetic nanoparticles and gel filtration, at 4°C, to homogeneity, 515fold with 73% yield | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.2 | plasma | - |
Homo sapiens | - |
3.1.8.1 | plasma | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.8.1 | 0.85 | - |
plasma, at 37°C | Homo sapiens |
3.1.8.1 | 438.2 | - |
515fold purified enzyme, at 37°C | Homo sapiens |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.8.1 | 4°C, 20% glycerol | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.2 | phenyl acetate + H2O | - |
Homo sapiens | phenol + acetate | - |
? | |
3.1.8.1 | paraoxon + H2O | - |
Homo sapiens | 4-nitrophenol + diethyl phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.2 | ? | x * 45000, SDS-PAGE | Homo sapiens |
3.1.8.1 | ? | x * 45000, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.8.1 | paraoxonase 1 | - |
Homo sapiens |
3.1.8.1 | PON1 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.2 | 37 | - |
- |
Homo sapiens |
3.1.8.1 | 37 | - |
- |
Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.8.1 | 37 | - |
is stable for 8 to 10 h at 37°C. Completely loses activity at 50°C | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.2 | 8 | - |
- |
Homo sapiens |
3.1.8.1 | 8 | - |
- |
Homo sapiens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.8.1 | additional information | - |
activity is reduced below pH 7.0 and at alkaline pH | Homo sapiens |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.2 | Homo sapiens | purified PON1, isoelectric focusing | - |
5.3 |
3.1.8.1 | Homo sapiens | purified PON1, isoelectric focusing | - |
5.3 |