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Literature summary extracted from

  • de Lima Damasio, A.R.; da Silva, T.M.; Maller, A.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.d.e.L.T.d.M.
    Purification and partial characterization of an exo-polygalacturonase from Paecilomyces variotii liquid cultures (2010), Appl. Biochem. Biotechnol., 160, 1496-1507.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 Ag+ 50% inhibition at 1 mM, complete inhibition at 10 mM Paecilomyces variotii
3.2.1.67 Al3+ inhibitory at 10 mM Paecilomyces variotii
3.2.1.67 Co2+ inhibitory at 10 mM Paecilomyces variotii
3.2.1.67 Fe3+ inhibitory at 10 mM Paecilomyces variotii
3.2.1.67 Hg2+ inhibitory at 10 mM Paecilomyces variotii
3.2.1.67 Mg2+ inhibitory at 10 mM Paecilomyces variotii
3.2.1.67 Mn2+ inhibitory at 10 mM Paecilomyces variotii
3.2.1.67 additional information no inhibition by Cu2+ and Na+, poor inhibition by K+ Paecilomyces variotii
3.2.1.67 Pb2+ inhibitory at 10 mM Paecilomyces variotii
3.2.1.67 Zn2+ inhibitory at 10 mM Paecilomyces variotii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 additional information
-
 additional information kinetics Paecilomyces variotii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 extracellular
-
 Paecilomyces variotii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 77300
-
 gel filtration Paecilomyces variotii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.67 pectin + H2O Paecilomyces variotii the enzyme is highly specific for pectin substrates, overview. It hydrolyzes specifically the terminal glycosidic bond of the polymer and releases galacturonic acid the only degradation product galacturonic acid + ?
-
 ?
3.2.1.67 polygalacturonic acid + H2O Paecilomyces variotii
-
 galacturonic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Paecilomyces variotii
-
 isolated from decaying hemicellulosic material collected in the state of Sao Paulo, Brazil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 native extracellular enzyme 13fold by anion exchange chromatography and gel filtration to homogeneity Paecilomyces variotii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.67 298.5
-
 purified native enzyme Paecilomyces variotii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 citric pectin + H2O the enzyme is highly specific for pectin substrates, citric pectins with a degree of esterification of 31%, 63%, and 93% result in 42.7%, 8.3%, and 0.0% relative activity rates, respectively, overview. It hydrolyzes specifically the terminal glycosidic bond of the polymer and releases galacturonic acid the only degradation product Paecilomyces variotii galacturonic acid + ?
-
 ?
3.2.1.67 additional information no activity with xylan, overview Paecilomyces variotii ?
-
 ?
3.2.1.67 pectin + H2O the enzyme is highly specific for pectin substrates, overview. It hydrolyzes specifically the terminal glycosidic bond of the polymer and releases galacturonic acid the only degradation product Paecilomyces variotii galacturonic acid + ?
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Paecilomyces variotii galacturonic acid + ?
-
 ?
3.2.1.67 sodium polypectate + H2O
-
 Paecilomyces variotii galacturonic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 monomer 1 * 77300, SDS-PAGE Paecilomyces variotii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exo-polygalacturonase
-
 Paecilomyces variotii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 65
-
-
 Paecilomyces variotii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 45
-
 purified enzyme, 90 min, completely stable Paecilomyces variotii
3.2.1.67 55
-
 purified enzyme, half-life is 50.6 min Paecilomyces variotii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 4
-
-
 Paecilomyces variotii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 3 6 purified enzyme, stable Paecilomyces variotii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.67 Paecilomyces variotii isoelectric focusing
-
 4.37