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Literature summary extracted from

  • Kim, D.J.; Kim, O.; Kim, H.W.; Kim, H.S.; Lee, S.J.; Suh, S.W.
    ATP-dependent DNA ligase from Archaeoglobus fulgidus displays a tightly closed conformation (2009), Acta Crystallogr. Sect. F, 65, 544-550.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expressed in Escherichia coli Rosetta2(DE3)pLysS cells Archaeoglobus fulgidus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.5.1.1 ATP-dependent DNA ligase in the DNA-unbound unadenylated state, hanging drop vapor diffusion method, using 100 mM Tris-HCl pH 9.0, 0.4 M sodium dihydrogen phosphate, 1.2 M dipotassium hydrogen phosphate, and 10 mM magnesium chloride, at 4°C Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Archaeoglobus fulgidus
-
 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Archaeoglobus fulgidus O29632
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 Ni-NTA agarose column chromatography, Source 15Q ion-exchange column chromatography, and Superdex-200 gel filtration Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Archaeoglobus fulgidus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 ATP-dependent DNA ligase
-
 Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.1 ATP
-
 Archaeoglobus fulgidus