Literature summary extracted from
Ishida, T.; Fujimoto, Z.; Ichinose, H.; Igarashi, K.; Kaneko, S.; Samejima, M.
Crystallization of selenomethionyl exo-beta-1,3-galactanase from the basidiomycete Phanerochaete chrysosporium (2009), Acta Crystallogr. Sect. F, 65, 1274-1276.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.145 |
Pc1,3Gal43A, expression in Pichia pastoris as selenmethionine-labeled protein |
Phanerodontia chrysosporium |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.2.1.145 |
selenomethionine-labeled recombinant Pc1,3Gal43A, hanging-drop vapour-diffusion method, 25°C, the reservoir solution contains 16% w/v PEG 10000, 95 mM ammonium sulfate, 95 mM Bis-Tris, pH 5.5, and 4.8% v/v glycerol, 3-10 days, X-ray diffraction structure determination and analysis at 1.8 A resolution, multiwavelength anomalous dispersion method, since molecular replacement is unsuccessful |
Phanerodontia chrysosporium |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.145 |
Phanerodontia chrysosporium |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.145 |
recombinant selenmethionine-labeled Pc1,3Gal43A from Pichia pastoris by ultrafiltration, and hydrophobic interaction chromatography |
Phanerodontia chrysosporium |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.145 |
More |
the enzyme consists of a glycoside hydrolase family 43 catalytic domain and a substrate-binding domain that belongs to carbohydrate-binding module family 35 |
Phanerodontia chrysosporium |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.145 |
exo-beta-1,3-galactanase |
- |
Phanerodontia chrysosporium |
3.2.1.145 |
More |
the enzyme consists of a glycoside hydrolase family 43 catalytic domain and a substrate-binding domain that belongs to carbohydrate-binding module family 35 |
Phanerodontia chrysosporium |
3.2.1.145 |
Pc1,3Gal43A |
- |
Phanerodontia chrysosporium |