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Literature summary extracted from

  • Wang, Y.; Meng, F.; Zhang, Y.
    Expression, purification and characterization of recombinant protein tyrosine phosphatase from Thermus thermophilus HB27 (2009), Acta Biochim. Biophys. Sin. (Shanghai), 41, 689-698.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.48 expression of the active and soluble PTPase in Escherichia coli strain BL21(DE3) Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.48 Cl-
-
 Thermus thermophilus
3.1.3.48 Cu2+
-
 Thermus thermophilus
3.1.3.48 SO42-
-
 Thermus thermophilus
3.1.3.48 Zn2+
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.48 6.01
-
 4-nitrophenyl phosphate pH 3.8, 75°C, recombinant enzyme Thermus thermophilus
3.1.3.48 37.88
-
 4-nitrophenyl phosphate pH 3.8, 30°C, recombinant enzyme Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.48 Ba2+ activates the recombinant enzyme Thermus thermophilus
3.1.3.48 Ca2+ activates the recombinant enzyme Thermus thermophilus
3.1.3.48 Mg2+ activates the recombinant enzyme Thermus thermophilus
3.1.3.48 Mn2+ activates 3fold at 5 mM, recombinant enzyme Thermus thermophilus
3.1.3.48 Ni2+ activates the recombinant enzyme Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.48 22000
-
 x * 22000, recombinant enzyme, SDS-PAGE, x * 22260, sequence calculation Thermus thermophilus
3.1.3.48 22260
-
 x * 22000, recombinant enzyme, SDS-PAGE, x * 22260, sequence calculation Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.48 Thermus thermophilus Q72JF6
-
-
3.1.3.48 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72JF6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.48 recombinant soluble PTPase 54.3fold from Escherichia coli strain BL21(DE3) by heat treatment and gel filtration to over 95% purity Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.48 5.02
-
 purified recombinant enzyme Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Thermus thermophilus 4-nitrophenol + phosphate
-
 ?
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 4-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.48 ? x * 22000, recombinant enzyme, SDS-PAGE, x * 22260, sequence calculation Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.48 low-molecular-weight protein tyrosine phosphatase
-
 Thermus thermophilus
3.1.3.48 PTPase
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.48 75
-
 recombinant enzyme Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.48 30 90 over 50% of maximal activity at 50-80°C, temperature profile, overview Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.48 50
-
 4 h, purified recombinant enzyme, no significant decrease in activity Thermus thermophilus
3.1.3.48 60
-
 4 h, purified recombinant enzyme, no significant decrease in activity Thermus thermophilus
3.1.3.48 70
-
 4 h, purified recombinant enzyme, loss of 34% activity Thermus thermophilus
3.1.3.48 80
-
 1 h, purified recombinant enzyme, 30% activity remaining Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.48 670.9
-
 4-nitrophenyl phosphate pH 3.8, 30°C, recombinant enzyme Thermus thermophilus
3.1.3.48 4013
-
 4-nitrophenyl phosphate pH 3.8, 75°C, recombinant enzyme Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.48 2.8 4.8 recombinant enzyme Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.48 2.2 10 pH profile, overview Thermus thermophilus

General Information

EC Number General Information Comment Organism
3.1.3.48 physiological function the heat-resistant PTPase may play important roles in vivo in the adaptation of the microorganism to extreme temperatures and specific nutritional conditions Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.48 17.71
-
 4-nitrophenyl phosphate pH 3.8, 30°C, recombinant enzyme Thermus thermophilus
3.1.3.48 667.8
-
 4-nitrophenyl phosphate pH 3.8, 75°C, recombinant enzyme Thermus thermophilus