EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.74 | using the crstal structure with PDB ID 1BQ6 for structure-function analysis, overview | Medicago sativa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.74 | F215S | the mutation does not significantly alter the Kd for CoA or acetyl-CoA binding, but dramatically alters the turnover rates for malonyl-CoA decarboxylation compared to the wild-type enzyme | Medicago sativa |
2.3.1.74 | F215Y | the mutation does not significantly alter the Kd for CoA or acetyl-CoA binding, but dramatically alters the turnover rates for malonyl-CoA decarboxylation compared to the wild-type enzyme | Medicago sativa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.74 | 3 malonyl-CoA + 4-coumaroyl-CoA | Medicago sativa | - |
4 CoA + naringenin chalcone + 3 CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.74 | Medicago sativa | P30074 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.74 | 3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2 | reaction mechanism analysis by quantum mechanics calculations, overview. In loading step, only a tetrahedral intermediate is located without transition state. His303 acts as a H31 donor, but not a hydrogen bond donor, to stabilize the intermediate formation. In decarboxylation step, the reaction proceeds via a transition state and is sensitive to the environment. In elongation step, a tetrahedral transition state is located, structure-function analysis, overview | Medicago sativa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.74 | 3 malonyl-CoA + 4-coumaroyl-CoA | - |
Medicago sativa | 4 CoA + naringenin chalcone + 3 CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.74 | chalcone synthase | - |
Medicago sativa |
2.3.1.74 | CHS | - |
Medicago sativa |