BRENDA - Enzyme Database

Quinoline 2-oxidoreductase and 2-oxo-1,2-dihydroquinoline 5,6-dioxygenase from Comamonas testosteroni 63. The first two enzymes in quinoline and 3-methylquinoline degradation

Schach, S.; Tshisuaka, B.; Fetzner, S.; Lingens, F.; Eur. J. Biochem. 232, 536-544 (1995)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.14.12.16
dithioerythritol, dithiothreitol and 2-oxo-1,2-dihydroquinoline stabilize
Comamonas testosteroni
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.12.16
1,10-phenanthroline
0.5 mM, 82% loss of activity
Comamonas testosteroni
1.14.12.16
4-hydroxymercuribenzoate
0.1 mM, complete loss of activity
Comamonas testosteroni
1.14.12.16
Acriflavin
0.5 mM, 69% loss of activity
Comamonas testosteroni
1.14.12.16
Cu2+
-
Comamonas testosteroni
1.14.12.16
diethyldithiocarbaminate
inhibits after a prolonged incubation time
Comamonas testosteroni
1.14.12.16
EDTA
inhibits after a prolonged incubation time
Comamonas testosteroni
1.14.12.16
iodoacetate
2 mM, complete loss of activity
Comamonas testosteroni
1.14.12.16
NaCl
0.2 M, 50% loss of activity
Comamonas testosteroni
1.14.12.16
Quinacrine
0.5 mM, 15% loss of activity
Comamonas testosteroni
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.99.17
Fe
enzyme contains 7.7 mol iron and 7.5 mol acid labile sulfur per mol of enzyme
Comamonas testosteroni
1.3.99.17
Fe
-
Pseudomonas putida
1.3.99.17
Fe
-
Rhodococcus sp.
1.3.99.17
Mo
molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Pseudomonas putida
1.3.99.17
Mo
molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Rhodococcus sp.
1.3.99.17
Mo
contains 1.6 mol molybdenum per mol of enzyme; molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Comamonas testosteroni
1.14.12.16
Fe2+
enhances activity 1.5fold
Comamonas testosteroni
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.99.17
22000
-
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE
Comamonas testosteroni
1.3.99.17
32000
-
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE
Comamonas testosteroni
1.3.99.17
87000
-
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE
Comamonas testosteroni
1.3.99.17
360000
-
gel filtration
Comamonas testosteroni
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.3.99.17
(3-Methyl)-quinoline + acceptor + H2O
Comamonas testosteroni
first step of 3-methylquinoline degradation
?
-
-
-
1.3.99.17
Quinoline + acceptor + H2O
Comamonas testosteroni
first step of quinoline degradation
?
-
-
-
1.14.12.16
additional information
Comamonas testosteroni
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
?
-
-
-
1.14.12.16
additional information
Comamonas testosteroni 63
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
?
-
-
-
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
1.14.12.16
Ethanol
5%, decreases enzyme activity to 44%
Comamonas testosteroni
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.3.99.17
Comamonas testosteroni
-
-
-
1.3.99.17
Comamonas testosteroni 63
-
-
-
1.3.99.17
Pseudomonas putida
-
-
-
1.3.99.17
Pseudomonas putida 86
-
-
-
1.3.99.17
Rhodococcus sp.
-
-
-
1.3.99.17
Rhodococcus sp. B1
-
-
-
1.14.12.16
Comamonas testosteroni
-
-
-
1.14.12.16
Comamonas testosteroni 63
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.3.99.17
-
Comamonas testosteroni
1.14.12.16
partial
Comamonas testosteroni
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.99.17
4.8
-
-
Comamonas testosteroni
1.14.12.16
0.125
-
-
Comamonas testosteroni
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.3.99.17
(3-methyl)-quinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida
(3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet
-
-
-
-
1.3.99.17
(3-methyl)-quinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Comamonas testosteroni
(3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet
-
7068
Comamonas testosteroni
-
1.3.99.17
(3-methyl)-quinoline + acceptor
61% of the activity with quinoline
7068
Comamonas testosteroni
(3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet
-
7068
Comamonas testosteroni
-
1.3.99.17
(3-methyl)-quinoline + acceptor
22% of the activity with quinoline
7068
Pseudomonas putida
(3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet
-
-
-
-
1.3.99.17
(3-Methyl)-quinoline + acceptor + H2O
first step of 3-methylquinoline degradation
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
4-chloroquinoline + p-iodonitrotetrazolium violet
5% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
4-chloroquinoline + p-iodonitrotetrazolium violet
8% of the activity with quinoline
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
4-methylquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
4-methylquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
4-methylquinoline + acceptor
21% of the activity with quinoline
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
4-methylquinoline + acceptor
12% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
58% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
25% of the activity with quinoline
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida 86
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
25% of the activity with quinoline
7068
Pseudomonas putida 86
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
55% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
29% of the activity with quinoline
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida 86
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
29% of the activity with quinoline
7068
Pseudomonas putida 86
?
-
-
-
-
1.3.99.17
8-methylquinoline + acceptor
32% of the activity with quinoline
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
8-methylquinoline + acceptor
54% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
isoquinoline + p-iodonitrotetrazolium violet
75% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
quinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida
2-oxo-1,2-dihydroquinoline + reduced acceptor
-
7068
Pseudomonas putida
-
1.3.99.17
quinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Comamonas testosteroni
2-oxo-1,2-dihydroquinoline + reduced acceptor
-
7068
Comamonas testosteroni
-
1.3.99.17
quinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida 86
2-oxo-1,2-dihydroquinoline + reduced acceptor
-
7068
Pseudomonas putida 86
-
1.3.99.17
Quinoline + acceptor + H2O
first step of quinoline degradation
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
2-Oxo-1,2-dihydroquinoline + NADH + O2
-
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
2-Oxo-1,2-dihydroquinoline + NADH + O2
-
7068
Comamonas testosteroni 63
?
-
-
-
-
1.14.12.16
3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2
NADH cannot be replaced by NADPH
7068
Comamonas testosteroni
5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+
-
7068
Comamonas testosteroni
-
1.14.12.16
3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2
NADH cannot be replaced by NADPH
7068
Comamonas testosteroni 63
5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+
-
7068
Comamonas testosteroni 63
-
1.14.12.16
6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
-
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
-
7068
Comamonas testosteroni 63
?
-
-
-
-
1.14.12.16
8-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
-
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
8-Hydroxyquinoline + NADH + O2
-
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
8-Hydroxyquinoline + NADH + O2
-
7068
Comamonas testosteroni 63
?
-
-
-
-
1.14.12.16
additional information
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
additional information
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
7068
Comamonas testosteroni 63
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.3.99.17
?
-
Pseudomonas putida
1.3.99.17
?
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE
Comamonas testosteroni
1.3.99.17
?
-
Rhodococcus sp.
1.3.99.17
More
the N-termini of the beta-subunits show striking similarities
Comamonas testosteroni
1.3.99.17
More
the N-termini of the beta-subunits show striking similarities
Pseudomonas putida
1.3.99.17
More
the N-termini of the beta-subunits show striking similarities
Rhodococcus sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.12.16
30
-
5 min, 30% loss of activity
Comamonas testosteroni
1.14.12.16
65
-
5 min, complete loss of activity
Comamonas testosteroni
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.12.16
7.3
-
-
Comamonas testosteroni
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.99.17
FAD
-
Pseudomonas putida
1.3.99.17
FAD
enzyme contains 1.8 mol FAD per mol of enzyme
Comamonas testosteroni
1.3.99.17
FAD
enzyme contains 1.8 mol FAD per mol of enzyme
Rhodococcus sp.
1.3.99.17
molybdopterin
molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Pseudomonas putida
1.3.99.17
molybdopterin
molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Rhodococcus sp.
1.3.99.17
molybdopterin
molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Comamonas testosteroni
1.14.12.16
NADH
required, cannot be replaced by NADPH
Comamonas testosteroni
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.99.17
FAD
enzyme contains 1.8 mol FAD per mol of enzyme
Comamonas testosteroni
1.3.99.17
FAD
-
Pseudomonas putida
1.3.99.17
FAD
enzyme contains 1.8 mol FAD per mol of enzyme
Rhodococcus sp.
1.3.99.17
molybdopterin
molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Pseudomonas putida
1.3.99.17
molybdopterin
molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Rhodococcus sp.
1.3.99.17
molybdopterin
molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Comamonas testosteroni
1.14.12.16
NADH
required, cannot be replaced by NADPH
Comamonas testosteroni
General Stability (protein specific)
EC Number
General Stability
Organism
1.14.12.16
dithioerythritol, dithiothreitol and 2-oxo-1,2-dihydroquinoline stabilize
Comamonas testosteroni
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.12.16
1,10-phenanthroline
0.5 mM, 82% loss of activity
Comamonas testosteroni
1.14.12.16
4-hydroxymercuribenzoate
0.1 mM, complete loss of activity
Comamonas testosteroni
1.14.12.16
Acriflavin
0.5 mM, 69% loss of activity
Comamonas testosteroni
1.14.12.16
Cu2+
-
Comamonas testosteroni
1.14.12.16
diethyldithiocarbaminate
inhibits after a prolonged incubation time
Comamonas testosteroni
1.14.12.16
EDTA
inhibits after a prolonged incubation time
Comamonas testosteroni
1.14.12.16
iodoacetate
2 mM, complete loss of activity
Comamonas testosteroni
1.14.12.16
NaCl
0.2 M, 50% loss of activity
Comamonas testosteroni
1.14.12.16
Quinacrine
0.5 mM, 15% loss of activity
Comamonas testosteroni
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.99.17
Fe
enzyme contains 7.7 mol iron and 7.5 mol acid labile sulfur per mol of enzyme
Comamonas testosteroni
1.3.99.17
Fe
-
Pseudomonas putida
1.3.99.17
Fe
-
Rhodococcus sp.
1.3.99.17
Mo
molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Pseudomonas putida
1.3.99.17
Mo
molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Rhodococcus sp.
1.3.99.17
Mo
contains 1.6 mol molybdenum per mol of enzyme; molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor
Comamonas testosteroni
1.14.12.16
Fe2+
enhances activity 1.5fold
Comamonas testosteroni
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.99.17
22000
-
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE
Comamonas testosteroni
1.3.99.17
32000
-
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE
Comamonas testosteroni
1.3.99.17
87000
-
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE
Comamonas testosteroni
1.3.99.17
360000
-
gel filtration
Comamonas testosteroni
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.3.99.17
(3-Methyl)-quinoline + acceptor + H2O
Comamonas testosteroni
first step of 3-methylquinoline degradation
?
-
-
-
1.3.99.17
Quinoline + acceptor + H2O
Comamonas testosteroni
first step of quinoline degradation
?
-
-
-
1.14.12.16
additional information
Comamonas testosteroni
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
?
-
-
-
1.14.12.16
additional information
Comamonas testosteroni 63
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
?
-
-
-
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
1.14.12.16
Ethanol
5%, decreases enzyme activity to 44%
Comamonas testosteroni
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.99.17
-
Comamonas testosteroni
1.14.12.16
partial
Comamonas testosteroni
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.99.17
4.8
-
-
Comamonas testosteroni
1.14.12.16
0.125
-
-
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.3.99.17
(3-methyl)-quinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida
(3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet
-
-
-
-
1.3.99.17
(3-methyl)-quinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Comamonas testosteroni
(3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet
-
7068
Comamonas testosteroni
-
1.3.99.17
(3-methyl)-quinoline + acceptor
61% of the activity with quinoline
7068
Comamonas testosteroni
(3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet
-
7068
Comamonas testosteroni
-
1.3.99.17
(3-methyl)-quinoline + acceptor
22% of the activity with quinoline
7068
Pseudomonas putida
(3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet
-
-
-
-
1.3.99.17
(3-Methyl)-quinoline + acceptor + H2O
first step of 3-methylquinoline degradation
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
4-chloroquinoline + p-iodonitrotetrazolium violet
5% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
4-chloroquinoline + p-iodonitrotetrazolium violet
8% of the activity with quinoline
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
4-methylquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
4-methylquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
4-methylquinoline + acceptor
21% of the activity with quinoline
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
4-methylquinoline + acceptor
12% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
58% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
25% of the activity with quinoline
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida 86
?
-
-
-
-
1.3.99.17
6-hydroxyquinoline + acceptor
25% of the activity with quinoline
7068
Pseudomonas putida 86
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
55% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
29% of the activity with quinoline
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida 86
?
-
-
-
-
1.3.99.17
8-chloroquinoline + acceptor
29% of the activity with quinoline
7068
Pseudomonas putida 86
?
-
-
-
-
1.3.99.17
8-methylquinoline + acceptor
32% of the activity with quinoline
7068
Pseudomonas putida
?
-
-
-
-
1.3.99.17
8-methylquinoline + acceptor
54% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
isoquinoline + p-iodonitrotetrazolium violet
75% of the activity with quinoline
7068
Comamonas testosteroni
?
-
-
-
-
1.3.99.17
quinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida
2-oxo-1,2-dihydroquinoline + reduced acceptor
-
7068
Pseudomonas putida
-
1.3.99.17
quinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Comamonas testosteroni
2-oxo-1,2-dihydroquinoline + reduced acceptor
-
7068
Comamonas testosteroni
-
1.3.99.17
quinoline + acceptor
acceptor: p-iodonitrotetrazolium violet
7068
Pseudomonas putida 86
2-oxo-1,2-dihydroquinoline + reduced acceptor
-
7068
Pseudomonas putida 86
-
1.3.99.17
Quinoline + acceptor + H2O
first step of quinoline degradation
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
2-Oxo-1,2-dihydroquinoline + NADH + O2
-
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
2-Oxo-1,2-dihydroquinoline + NADH + O2
-
7068
Comamonas testosteroni 63
?
-
-
-
-
1.14.12.16
3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2
NADH cannot be replaced by NADPH
7068
Comamonas testosteroni
5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+
-
7068
Comamonas testosteroni
-
1.14.12.16
3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2
NADH cannot be replaced by NADPH
7068
Comamonas testosteroni 63
5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+
-
7068
Comamonas testosteroni 63
-
1.14.12.16
6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
-
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
-
7068
Comamonas testosteroni 63
?
-
-
-
-
1.14.12.16
8-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
-
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
8-Hydroxyquinoline + NADH + O2
-
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
8-Hydroxyquinoline + NADH + O2
-
7068
Comamonas testosteroni 63
?
-
-
-
-
1.14.12.16
additional information
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
7068
Comamonas testosteroni
?
-
-
-
-
1.14.12.16
additional information
second enzyme in the pathway of quinoline and 3-methylquinoline degradation
7068
Comamonas testosteroni 63
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.99.17
?
-
Pseudomonas putida
1.3.99.17
?
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE
Comamonas testosteroni
1.3.99.17
?
-
Rhodococcus sp.
1.3.99.17
More
the N-termini of the beta-subunits show striking similarities
Comamonas testosteroni
1.3.99.17
More
the N-termini of the beta-subunits show striking similarities
Pseudomonas putida
1.3.99.17
More
the N-termini of the beta-subunits show striking similarities
Rhodococcus sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.12.16
30
-
5 min, 30% loss of activity
Comamonas testosteroni
1.14.12.16
65
-
5 min, complete loss of activity
Comamonas testosteroni
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.12.16
7.3
-
-
Comamonas testosteroni