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Literature summary extracted from

  • OuYang, B.; Pochapsky, S.S.; Dang, M.; Pochapsky, T.C.
    A functional proline switch in cytochrome P450cam (2008), Structure, 16, 916-923.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.1 additional information involvement of X-proline isomerization in enzyme function Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.1 mutants overexpressed from Escherichia coli strain NCM533 harboring modified pDNC334A plasmids that encode the appropriate mutant of CYP101 under control of the lac promoter Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 C334A mutant, that is spectroscopically and enzymatically identical to wild-type CYP101 enzyme, but does not form dimers in solution, and so is more suitable for solution NMR studies than wild-type enzyme Pseudomonas putida
1.14.15.1 P89I yields a mixture of both bound camphor orientations, that seen in putidaredoxin-free and that seen in putidaredoxin-bound CYP101. A mutation in CYP101 that destabilizes the cis conformer of the Ile-88-Pro-89 amide bond results in weaker binding of putidaredoxin Pseudomonas putida
1.14.15.1 Y29F the cis conformer is destabilized by the absence of the hydrogen bond between the carbonyl oxygen of Ile-88 and the Tyr-29 hydroxyl group Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.1
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin conformational change in CYP101 upon binding of putidaredoxin that re-orients bound camphor appropriately for hydroxylation Pseudomonas putida exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 CYP101
-
 Pseudomonas putida
1.14.15.1 cytochrome p450cam
-
 Pseudomonas putida
1.14.15.1 P450cam
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 putidaredoxin binding of putidaredoxin converts a single X-proline amide bond in CYP101 from trans or distorted trans to cis Pseudomonas putida