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Literature summary extracted from

  • Schlaefli, H.R.; Weiss, M.A.; Leisinger, T.; Cook, A.M.
    Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component (1994), J. Bacteriol., 176, 6644-6652.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.15 Iron the enzyme is a Rieske [2Fe-2S] protein Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.15 18000
-
 2 * 49000, alpha, + 2 * 18000, beta, oxygenase component of the TER dioxygenase system, SDS-PAGE Comamonas testosteroni
1.14.12.15 49000
-
 2 * 49000, alpha, + 2 * 18000, beta, oxygenase component of the TER dioxygenase system, SDS-PAGE Comamonas testosteroni
1.14.12.15 126000
-
 oxygenase component of the TER dioxygenase system, gel filtration Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.15 Comamonas testosteroni
-
-
-
1.14.12.15 Comamonas testosteroni T-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.15 oxygenase component of the TER dioxygenase system. The reductase component or components cannot be purified Comamonas testosteroni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.12.15 culture condition:terephthalate-grown cell
-
 Comamonas testosteroni
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.15 1,4-Dicarboxynaphthalene + O2
-
 Comamonas testosteroni ?
-
 ?
1.14.12.15 1,4-Dicarboxynaphthalene + O2
-
 Comamonas testosteroni T-2 ?
-
 ?
1.14.12.15 2,5-Dicarboxypyridine + O2
-
 Comamonas testosteroni ?
-
 ?
1.14.12.15 2,5-Dicarboxypyridine + O2
-
 Comamonas testosteroni T-2 ?
-
 ?
1.14.12.15 terephthalate + NADH + O2 + H+
-
 Comamonas testosteroni (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
 ?
1.14.12.15 terephthalate + NADH + O2 + H+
-
 Comamonas testosteroni T-2 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.15 tetramer 2 * 49000, alpha, + 2 * 18000, beta, oxygenase component of the TER dioxygenase system, SDS-PAGE Comamonas testosteroni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.12.15 25 36 oxygenase component of the TER dioxygenase system Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.15 7.5 8.5 oxygenase component of the TER dioxygenase system Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.15 NADH
-
 Comamonas testosteroni