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Literature summary extracted from

  • Karmodiya, K.; Surolia, N.
    A unique and differential effect of denaturants on cofactor mediated activation of Plasmodium falciparum beta-ketoacyl-ACP reductase (2008), Proteins Struct. Funct. Genet., 70, 528-538.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.100 additional information affinity of acyl-carrier protein for FabG is increased by 3fold in the presence of 1 M urea Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.100 into pET-28a vector and expressed in Escherichia coli BL21 (DE3) codon plus Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.100 Guanidinium chloride FabG is fully unfolded at 4 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. In presence of NADPH, there is no stabilization of FabG in case of equilibrium unfolding with guanidinium chloride Plasmodium falciparum
1.1.1.100 Urea FabG is fully unfolded at 6 M, approximately 90% of the enzyme activity can be recovered on dialyzing the denaturant. Two states in the reversible unfolding process of FabG in presence of NADPH, one is an activity-enhanced state and the other, an inactive state in case of equilibrium unfolding with urea Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 0.0412
-
 NADPH in the presence of 1.5 M urea, pH 7.0, 25°C Plasmodium falciparum
1.1.1.100 0.0434
-
 NADPH in the presence of 1 M urea, pH 7.0, 25°C Plasmodium falciparum
1.1.1.100 0.0461
-
 NADPH without urea, pH 7.0, 25°C Plasmodium falciparum
1.1.1.100 0.4553
-
 NADPH in the presence of 4 M urea, pH 7.0, 25°C Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Plasmodium falciparum (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 acetoacetyl-CoA + NADPH
-
 Plasmodium falciparum 3-hydroxybutyryl-CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.100 tetramer cross-linking of native and up to 1 M urea-treated FabG Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.100 beta-ketoacyl-ACP reductase
-
 Plasmodium falciparum
1.1.1.100 FabG
-
 Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.100 148.6
-
 NADPH in the presence of 4 M urea, pH 7.0, 25°C Plasmodium falciparum
1.1.1.100 187.3
-
 NADPH in the presence of 1.5 M urea, pH 7.0, 25°C Plasmodium falciparum
1.1.1.100 250
-
 NADPH without urea, pH 7.0, 25°C Plasmodium falciparum
1.1.1.100 344.7
-
 NADPH in the presence of 1 M urea, pH 7.0, 25°C Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADPH
-
 Plasmodium falciparum