EC Number | Crystallization (Comment) | Organism |
---|---|---|
6.3.4.14 | in absence and presence of ATP. Upon ATP binding, the central B-domain closes. Residues G165 and G166 play a role in ATP binding | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.14 | G165V | the mutation does not affect the maximal velocity of a partial reaction, the bicarbonate-dependent ATPase activity. Km values for ATP increases over 40fold when compared with wild-type. The maximal velocity for the biotin-dependent ATPase activity, i.e. the complete reaction, decreases over 100fold | Escherichia coli |
6.3.4.14 | G165V/G166V | the mutation does not affect the maximal velocity of a partial reaction, the bicarbonate-dependent ATPase activity. Km values for ATP increases over 40fold when compared with wild-type. The maximal velocity for the biotin-dependent ATPase activity, i.e. the complete reaction, decreases over 100fold | Escherichia coli |
6.3.4.14 | G166V | the mutation does not affect the maximal velocity of a partial reaction, the bicarbonate-dependent ATPase activity. Km values for ATP increases over 40fold when compared with wild-type. The maximal velocity for the biotin-dependent ATPase activity, i.e. the complete reaction, decreases over 100fold | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.14 | 0.5 | - |
HCO3- | mutant G165V/G166V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 0.8 | - |
HCO3- | mutant G166V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 1.7 | - |
HCO3- | mutant G165V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 3.3 | - |
ATP | mutant G165V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 3.4 | - |
ATP | mutant G166V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 3.8 | - |
ATP | mutant G165V/G166V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 186 | - |
biotin | mutant G166V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 256 | - |
biotin | mutant G165V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 321 | - |
biotin | mutant G165V/G166V, pH 8.0, 25°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.14 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.14 | ATP + biotin + HCO3- | - |
Escherichia coli | ADP + phosphate + carboxybiotin | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.14 | 0.0016 | - |
HCO3- | mutant G165V/G166V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 0.0016 | - |
HCO3- | mutant G166V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 0.0025 | - |
HCO3- | mutant G165V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 0.0037 | - |
biotin | mutant G166V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 0.005 | - |
biotin | mutant G165V, pH 8.0, 25°C | Escherichia coli | |
6.3.4.14 | 0.007 | - |
biotin | mutant G165V/G166V, pH 8.0, 25°C | Escherichia coli |