Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yang, X.; Lewis, P.J.
    Overproduction and purification of recombinant Bacillus subtilis RNA polymerase (2008), Protein Expr. Purif., 59, 86-93.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.6 gene rpoA, recombinant expression of the C-terminally His9-tagged enzyme in Escherichia coli B834 (DE3) cells, subcloning in Escherichia coli strain DH5alpha Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Mg2+
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn Bacillus subtilis
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6 recombinant C-terminally His9-tagged enzyme to 90% purity by nickel affinity chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn
-
 Bacillus subtilis diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 RNA polymerase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.6 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 7.9
-
 assay at Bacillus subtilis