BRENDA - Enzyme Database show

Membrane-binding and enzymatic properties of RPE65

Kiser, P.D.; Palczewski, K.; Prog. Retin. Eye Res. 29, 428-442 (2010)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
cytosol
the ratio of cytosolic to microsomal RPE65 is approximately 1:2
Bos taurus
5829
-
3.1.1.64
membrane
microsome
Bos taurus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.64
Fe2+
required
Bos taurus
3.1.1.64
additional information
Fe3+, Cu2+, Mg2+ and Zn2+ are unable to restore RPE65 isomerase activity
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.64
65000
-
x * 65000, SDS-PAGE
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.64
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.64
purified from native bovine RPE microsomes in the presence of octyltetraoxyethylene. Triton X-100 and CHAPS are the most effective agents for extracting RPE65 from microsomal membranes
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelial cell
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinol
-
706574
Bos taurus
11-cis-retinol
-
-
-
?
3.1.1.64
all-trans-retinyl ester + H2O
-
706574
Bos taurus
11-cis-retinol + a fatty acid
-
-
-
?
3.1.1.64
additional information
all-trans-9-des-methylretinol, retinyl acetate, retinyl heptanoate, all-trans-11-fluororetinol and all-trans-19,19,19-trifluororetinol are not isomerized
706574
Bos taurus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.64
?
x * 65000, SDS-PAGE
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
cytosol
the ratio of cytosolic to microsomal RPE65 is approximately 1:2
Bos taurus
5829
-
3.1.1.64
membrane
microsome
Bos taurus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.64
Fe2+
required
Bos taurus
3.1.1.64
additional information
Fe3+, Cu2+, Mg2+ and Zn2+ are unable to restore RPE65 isomerase activity
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.64
65000
-
x * 65000, SDS-PAGE
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
purified from native bovine RPE microsomes in the presence of octyltetraoxyethylene. Triton X-100 and CHAPS are the most effective agents for extracting RPE65 from microsomal membranes
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelial cell
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinol
-
706574
Bos taurus
11-cis-retinol
-
-
-
?
3.1.1.64
all-trans-retinyl ester + H2O
-
706574
Bos taurus
11-cis-retinol + a fatty acid
-
-
-
?
3.1.1.64
additional information
all-trans-9-des-methylretinol, retinyl acetate, retinyl heptanoate, all-trans-11-fluororetinol and all-trans-19,19,19-trifluororetinol are not isomerized
706574
Bos taurus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.64
?
x * 65000, SDS-PAGE
Bos taurus
General Information
EC Number
General Information
Commentary
Organism
3.1.1.64
physiological function
RPE65 is involved not only for regeneration of rhodopsin but also plays an important role in regeneration of cone opsins and is critical for the health of cone photoreceptors. Interactions of RPE65 with an intact phospholipid bilayer are critical for its enzymatic activity
Bos taurus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.1.64
physiological function
RPE65 is involved not only for regeneration of rhodopsin but also plays an important role in regeneration of cone opsins and is critical for the health of cone photoreceptors. Interactions of RPE65 with an intact phospholipid bilayer are critical for its enzymatic activity
Bos taurus