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Literature summary extracted from

  • Ji, G.; Silver, S.
    Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of Staphylococcus aureus plasmid pI258 (1992), Proc. Natl. Acad. Sci. USA, 89, 9474-9478.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.20.4.4 dithiothreitol stimulates ArsC-dependent arsenate reduction, but not 2-mercaptoethanol or reduced glutathione Staphylococcus aureus subsp. aureus RN4220
1.20.4.4 additional information reduction of arsenate to arsenite is dependent on the presence of the arsC gene and the extent of reduction depended also upon the presence of arsB. The arsC-deletion mutant plasmids pGJ106 and pGJ107 confer no more arsenate reduction activity than does the vector plasmid pSK265. Without intact arsB (plasmids pGJ105 and pGJ109) somewhat less arsenite is found Staphylococcus aureus subsp. aureus RN4220
1.20.4.4 thioredoxin and dithiothreitol are needed for arsenate reduction Staphylococcus aureus subsp. aureus RN4220

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.4.4 overproduced in Escherichia coli K38(pGP1-2) Staphylococcus aureus subsp. aureus RN4220

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.20.4.4 iodoacetate sulfhydryl inhibitors N-ethylmaleimide -and iodoacetate inhibit arsenate reductase activity by 80% in crude cell-free preparations and by 90% with purified ArsC protein Staphylococcus aureus subsp. aureus RN4220
1.20.4.4 N-ethylmaleimide sulfhydryl inhibitors N-ethylmaleimide -and iodoacetate inhibit arsenate reductase activity by 80% in crude cell-free preparations and by 90% with purified ArsC protein Staphylococcus aureus subsp. aureus RN4220

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.20.4.4 cytoplasm
-
 Staphylococcus aureus subsp. aureus RN4220 5737
-

Organism

EC Number Organism UniProt Comment Textmining
1.20.4.4 Staphylococcus aureus subsp. aureus RN4220 P0A006
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.20.4.4 by fast protein liquid chromatography Staphylococcus aureus subsp. aureus RN4220

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.4.4 arsenate + thioredoxin
-
 Staphylococcus aureus subsp. aureus RN4220 arsenite + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.20.4.4 ArsC plasmid p1258 Staphylococcus aureus subsp. aureus RN4220
1.20.4.4 arsenate reductase
-
 Staphylococcus aureus subsp. aureus RN4220

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.20.4.4 37
-
 assay at Staphylococcus aureus subsp. aureus RN4220

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.20.4.4 80
-
 heat-stable, surviving 2 min heating at 80°C Staphylococcus aureus subsp. aureus RN4220

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.20.4.4 7 9.5 ArsC arsenate reductase activity is decreased >80% at pH 6.3 but is stable in the pH range 7.0-9.5 Staphylococcus aureus subsp. aureus RN4220

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.20.4.4 7 9.5 ArsC arsenate reductase activity is decreased >80% at pH 6.3 but is stable in the pH range 7.0-9.5 Staphylococcus aureus subsp. aureus RN4220