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Literature summary extracted from

  • Chang, C.W.; Guo, L.; Kao, Y.T.; Li, J.; Tan, C.; Li, T.; Saxena, C.; Liu, Z.; Wang, L.; Sancar, A.; Zhong, D.
    Ultrafast solvation dynamics at binding and active sites of photolyases (2010), Proc. Natl. Acad. Sci. USA, 107, 2914-2919.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.3 N341A life-time of 2.9 ns compared to 1.3 ns for the wild-type enzyme Escherichia coli
4.1.99.3 W395R fully binds methenyltetrahydrofolate but not fully reduced FADH-, life-time of 2.9 ns Caulobacter vibrioides

General Stability

EC Number General Stability Organism
4.1.99.3 life-time of wild-type enzyme is 1.3 ns Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.3 Caulobacter vibrioides
-
-
-
4.1.99.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.3
-
 Caulobacter vibrioides
4.1.99.3 purified with neutral radical FADH and/or oxidized FAD forms and then converted to a fully reduced FADH- form under anaerobic conditions Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.3 cyclobutadipyrimidine in DNA
-
 Caulobacter vibrioides ?
-
 ?
4.1.99.3 cyclobutadipyrimidine in DNA presence of a very rigid antenna binding site, a relatively rigid active site in CPD photolyase but with large local orientation flexibility Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.99.3 CPD photolyase
-
 Escherichia coli
4.1.99.3 CPD photolyase
-
 Caulobacter vibrioides
4.1.99.3 PHR
-
 Escherichia coli
4.1.99.3 PHR
-
 Caulobacter vibrioides

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.3 FADH2
-
 Caulobacter vibrioides
4.1.99.3 FADH2 heterogeneous dynamics continuously tune local configurations to optimize photolyase's function through resonance energy transfer from the antenna to the cofactor for energy efficiency and then electron transfer between the cofactor and the substrate for repair of damaged DNA Escherichia coli
4.1.99.3 methenyltetrahydrofolate
-
 Escherichia coli
4.1.99.3 methenyltetrahydrofolate
-
 Caulobacter vibrioides