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Literature summary extracted from

  • Li, C.; Lu, C.D.
    Arginine racemization by coupled catabolic and anabolic dehydrogenases (2009), Proc. Natl. Acad. Sci. USA, 106, 906-911.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.25 expression in Escherichia coli Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.9 D-arginine Pseudomonas aeruginosa racemization of arginine is a two-step mechanism, intermediate is 2-keto-arginine, a first enzyme (DauA) catalyzes oxidative deamination of D-arginine into 2-ketoarginine and ammonia, and the second enzyme DauB is able to use 2-ketoarginine and ammonia as substrates and convert them into L-arginine in the presence of NADPH or NADH, DauA and DauB are coupled catabolic and anabolic dehydrogenases to perform D-to-L racemization of arginine, which serves as prerequisite of D-arginine utilization through L-arginine catabolic pathways L-arginine
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.25 Pseudomonas aeruginosa Q9HXE4
-
-
1.4.1.25 Pseudomonas aeruginosa DSM 22644 Q9HXE4
-
-
1.4.99.6 Pseudomonas aeruginosa Q9HXE3
-
-
5.1.1.9 Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.25 5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+ DauB uses 2-ketoarginine and ammonia derived from D-arginine in the DauA-catalyzed reaction Pseudomonas aeruginosa L-arginine + H2O + NAD(P)+
-
 ?
1.4.1.25 5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+ DauB uses 2-ketoarginine and ammonia derived from D-arginine in the DauA-catalyzed reaction Pseudomonas aeruginosa DSM 22644 L-arginine + H2O + NAD(P)+
-
 ?
1.4.1.25 L-arginine + H2O + NAD(P)+
-
 Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+
-
 r
1.4.1.25 L-arginine + H2O + NAD(P)+
-
 Pseudomonas aeruginosa DSM 22644 5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+
-
 r
1.4.1.25 additional information no substantial reaction with D-arginine Pseudomonas aeruginosa ?
-
 ?
1.4.1.25 additional information no substantial reaction with D-arginine Pseudomonas aeruginosa DSM 22644 ?
-
 ?
5.1.1.9 D-arginine racemization of arginine is a two-step mechanism, intermediate is 2-keto-arginine, a first enzyme (DauA) catalyzes oxidative deamination of D-arginine into 2-ketoarginine and ammonia, and the second enzyme DauB is able to use 2-ketoarginine and ammonia as substrates and convert them into L-arginine in the presence of NADPH or NADH, DauA and DauB are coupled catabolic and anabolic dehydrogenases to perform D-to-L racemization of arginine, which serves as prerequisite of D-arginine utilization through L-arginine catabolic pathways Pseudomonas aeruginosa L-arginine
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.4.1.25 dauB
-
 Pseudomonas aeruginosa
1.4.1.25 NAD(P)H-dependent anabolic L-arginine dehydrogenase
-
 Pseudomonas aeruginosa
1.4.99.6 DauA
-
 Pseudomonas aeruginosa
5.1.1.9 arginine racemase
-
 Pseudomonas aeruginosa

Expression

EC Number Organism Comment Expression
1.4.1.25 Pseudomonas aeruginosa expression of the DauBAR operon is highly induced in presence of D-arginine up

General Information

EC Number General Information Comment Organism
1.4.1.25 physiological function strains with a lesion at DauA or DauB genes fail to use D-arginine as sole carbon source. Growth complementation of an L-arginine auxotroph by D-arginine is abolished by a lesion at DauA or DauB. Presence of D-arginine induces L-arginine-specific genes in the parental strain PAO1 but not in its dauA or dauB mutants. DauA catalyzes oxidative deamination of D-arginine into 2-ketoarginine and ammonia, and DauB is able to use 2-ketoarginine and ammonia as substrates and convert them into L-arginine in the presence of NADPH or NADH Pseudomonas aeruginosa
1.4.99.6 physiological function D-to-L inversion in D-arginine metabolism requires both D-arginine dehydrogenase DauA and NAD(P)H-dependent anabolic L-arginine dehydrogenase DauB. DauA catalyzes oxidative deamination of D-arginine into 2-oxoarginine and ammonia, and DauB is able to use 2-ketoarginine and ammonia as substrates and convert them into L-arginine in the presence of NADPH or NADH. DauA and DauB are coupled catabolic and anabolic dehydrogenases that enable D-arginine utilization through L-arginine catabolic pathways Pseudomonas aeruginosa