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Literature summary extracted from

  • Sorci, L.; Martynowski, D.; Rodionov, D.A.; Eyobo, Y.; Zogaj, X.; Klose, K.E.; Nikolaev, E.V.; Magni, G.; Zhang, H.; Osterman, A.L.
    Nicotinamide mononucleotide synthetase is the key enzyme for an alternative route of NAD biosynthesis in Francisella tularensis (2009), Proc. Natl. Acad. Sci. USA, 106, 3083-3088.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.1.5 to 1.9 A resolution Francisella tularensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.5 0.2
-
deamido-NMN
-
Francisella tularensis
6.3.1.5 5.82
-
deamido-NAD+
-
Francisella tularensis

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.5 Francisella tularensis A0A0B3VMT3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3
-
Francisella tularensis AMP + diphosphate + NAD+
-
?
6.3.1.5 ATP + deamido-NMN + NH3 existence of an alternative route of NAD synthesis where the amidation of NaMN to nicotinamide mononucleotide occurs before the adenylylation reaction, which converts the intermediate to the NAD cofactor. The first step is catalyzed by Francisella tularensis NadE Francisella tularensis AMP + diphosphate + NMN
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.1.5 0.25
-
deamido-NAD+
-
Francisella tularensis
6.3.1.5 0.5
-
deamido-NMN
-
Francisella tularensis

General Information

EC Number General Information Comment Organism
6.3.1.5 physiological function existence of an alternative route of NAD synthesis where the amidation of NaMN to nicotinamide mononucleotide occurs before the adenylylation reaction, which converts the intermediate to the NAD cofactor. The first step is catalyzed by Francisella tularensis NadE Francisella tularensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.1.5 43
-
deamido-NAD+
-
Francisella tularensis
6.3.1.5 2500
-
deamido-NMN
-
Francisella tularensis