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Literature summary extracted from
- Identification and characterization of a super-stable Cu-Zn SOD from leaves of turmeric (Curcuma longa L.) (2008), Planta, 228, 307-318.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.15.1.1 | activity of the SOD remains intact both in crude and purified forms after autoclaving at 6-20 bars up to 10 min and microwaving at a frequency of 2450 MHz or million cycles per second for 1-3 min | Curcuma longa |
| 1.15.1.1 | polyvinylchloride stabilizes the enzyme | Curcuma longa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.15.1.1 | EDTA | inhibition is reversible by Cu and Zn | Curcuma longa |  |
| 1.15.1.1 | H2O2 | - |
Curcuma longa | |
| 1.15.1.1 | KCN | - |
Curcuma longa | |
| 1.15.1.1 | Sodium diethyldithiocarbamate | complete inhibition above 0.1 mM | Curcuma longa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.15.1.1 | chloroplast | - |
Curcuma longa | 9507 | - |
| 1.15.1.1 | cytoplasm | - |
Curcuma longa | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.15.1.1 | Cu | a Cu-Zn SOD, activates | Curcuma longa | |
| 1.15.1.1 | Zn | a Cu-Zn SOD | Curcuma longa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 15000 | - |
2 * 15000, SDS-PAGE | Curcuma longa |
| 1.15.1.1 | 30800 | - |
native PAGE | Curcuma longa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.15.1.1 | Curcuma longa | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.15.1.1 | leaf | - |
Curcuma longa | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.15.1.1 | 4°C, purified enzyme, stable for at least 18 months | Curcuma longa |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.15.1.1 | dimer | 2 * 15000, SDS-PAGE | Curcuma longa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.15.1.1 | Cu-Zn SOD | - |
Curcuma longa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 40 | - |
purified enzyme, completely stable after 20 min, after 1 h, 98% remaining activity of the leaf enzyme, 88% of the rhizome enzyme | Curcuma longa |
| 1.15.1.1 | 60 | - |
purified enzyme, completely stable after 20 min, after 1 h, 90% remaining activity of the leaf enzyme, 70% of the rhizome enzyme | Curcuma longa |
| 1.15.1.1 | 80 | - |
purified enzyme, completely stable after 20 min, after 1 h, 80% remaining activity of the leaf enzyme, 52% of the rhizome enzyme | Curcuma longa |
| 1.15.1.1 | 100 | - |
purified enzyme, loss of 5% activity after 10 min boiling for the chloroplast enzyme and 35% for the cytosolic enzyme, after 1 h, 70% remaining activity, of the leaf enzyme, 20% of the rhizome enzyme | Curcuma longa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 7 | 8 | assay at | Curcuma longa |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.15.1.1 | 0.04 | - |
- |
Curcuma longa | Sodium diethyldithiocarbamate | |
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