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Literature summary extracted from

  • Hwang, I.S.; Hwang, B.K.
    The pepper 9-lipoxygenase gene CaLOX1 functions in defense and cell death responses to microbial pathogens (2010), Plant Physiol., 152, 948-967.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.58 expressing full-length CaLOX1 in Escherichia coli as a fusion protein with an N-terminal His tag. Overexpression of CaLOX1 in Arabidopsis (Arabidopsis thaliana) conferres enhanced resistance to Pseudomonas syringae pv tomato, Hyaloperonospora arabidopsidis, and Alternaria brassicicola Capsicum annuum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.58 0.0203
-
 arachidonate pH 6.5, 25°C Capsicum annuum
1.13.11.58 0.114
-
 linoleate pH 6.5, 25°C Capsicum annuum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.58 linoleate + O2 Capsicum annuum
-
 (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
 ?
1.13.11.58 linoleate + O2 Capsicum annuum Nockwang
-
 (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.58 Capsicum annuum D3TTH9 Nockwang
-
1.13.11.58 Capsicum annuum Nockwang D3TTH9 Nockwang
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.58 leaf expression of CaLOX1 is differentially induced in pepper leaves not only during Xanthomonas campestris pv. vesicatoria infection but also after exposure to abiotic elicitors. Transient expression of CaLOX1 in pepper leaves induces the cell death phenotype and defense responses Capsicum annuum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.58 arachidonate + O2
-
 Capsicum annuum ?
-
 ?
1.13.11.58 arachidonate + O2
-
 Capsicum annuum Nockwang ?
-
 ?
1.13.11.58 linoleate + O2
-
 Capsicum annuum (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
 ?
1.13.11.58 linoleate + O2
-
 Capsicum annuum (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate no formation of 13-hydroperoxy-(10E,12Z)-octadecadienoate. The R/S stereoconfiguration of the product is not determined ?
1.13.11.58 linoleate + O2
-
 Capsicum annuum Nockwang (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
 ?
1.13.11.58 linoleate + O2
-
 Capsicum annuum Nockwang (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate no formation of 13-hydroperoxy-(10E,12Z)-octadecadienoate. The R/S stereoconfiguration of the product is not determined ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.58 9-lipoxygenase
-
 Capsicum annuum
1.13.11.58 9-LOX
-
 Capsicum annuum
1.13.11.58 CaLOX1
-
 Capsicum annuum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.58 25
-
-
 Capsicum annuum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.58 1.3
-
 arachidonate pH 6.5, 25°C Capsicum annuum
1.13.11.58 8.7
-
 linoleate pH 6.5, 25°C Capsicum annuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.58 6 6.5
-
 Capsicum annuum

Expression

EC Number Organism Comment Expression
1.13.11.58 Capsicum annuum expression of CaLOX1 is induced 1 h after infection by the virulent strain Ds1, peaks at 20 h, and decreased thereafter. Expression of CaLOX1 is significantly increased by mock inoculation. The induction of CaLOX1 transcript in mock-inoculated or virulent Xcv inoculated leaves may be due, in part, to the wounding effect. Treatment with ethylene, SA, NaCl, and methyl viologen significantly induces CaLOX1 in pepper leaves up

General Information

EC Number General Information Comment Organism
1.13.11.58 malfunction CaLOX1-silenced pepper plants are more susceptible to Xanthomonas campestris pv. vesicatoria and Colletotrichum coccodes infection Capsicum annuum
1.13.11.58 physiological function CaLOX1 positively regulates defense and cell death responses to microbial pathogens Capsicum annuum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.58 0.062
-
 arachidonate pH 6.5, 25°C Capsicum annuum
1.13.11.58 0.076
-
 linoleate pH 6.5, 25°C Capsicum annuum