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Literature summary extracted from

  • Ruiz-Lopez, N.; Garces, R.; Harwood, J.L.; Martinez-Force, E.
    Characterization and partial purification of acyl-CoA:glycerol 3-phosphate acyltransferase from sunflower (Helianthus annuus L.) developing seeds (2009), Plant Physiol. Biochem., 48, 73-80.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 EDTA 10 mM, enhances activity, relative activity: 143% Helianthus annuus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 Mg2+ 2 mM, 87% residual activity; slightly decreases activity, relative activity: 87.5% (2 mM), 37% (5 mM) Helianthus annuus
2.3.1.15 Mn2+ 2 mM, 82% residaul activity; slightly decreases activity, relative activity: 81.5% (2 mM) Helianthus annuus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.354
-
glycerol 3-phosphate
-
Helianthus annuus
2.3.1.15 0.354
-
glycerol 3-phosphate pH 7.5, 30°C Helianthus annuus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 microsome
-
Helianthus annuus
-
-
2.3.1.15 microsome GPAT is prepared from microsomes Helianthus annuus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 60100
-
SDS-PAGE Helianthus annuus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Helianthus annuus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 high solubilisation is achieved using 0.75% Tween80 and the solubilised GPAT is partially purified by ion-exchange chromatography using a Hi-Trap DEAE FF column, followed by gel filtration chromatography using a Superose 12 HR column Helianthus annuus
2.3.1.15 partial Helianthus annuus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 seed
-
Helianthus annuus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.15 additional information
-
maximum value for GPAT activity identified between 15 and 20 days after flowering Helianthus annuus
2.3.1.15 0.037
-
pH 7.5, 30°C Helianthus annuus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 linoleoyl-CoA + glycerol 3-phosphate preferred substrate linoleoyl-CoA (equal to palmitoyl-CoA) Helianthus annuus CoA + 1-linoleoyl-glycerol 3-phosphate
-
?
2.3.1.15 linoleoyl-CoA + glycerol 3-phosphate and palmitoyl-CoA, best substrates Helianthus annuus CoA + 1-linoleoyl-glycerol 3-phosphate
-
?
2.3.1.15 oleoyl-CoA + glycerol 3-phosphate
-
Helianthus annuus CoA + 1-oleoyl-glycerol 3-phosphate
-
?
2.3.1.15 oleoyl-CoA + glycerol 3-phosphate moderate activity with oleoyl-CoA Helianthus annuus CoA + 1-oleoyl-glycerol 3-phosphate
-
?
2.3.1.15 palmitoyl-CoA + glycerol 3-phosphate preferred substrate palmitoyl-CoA (equal to linoleoyl-CoA) Helianthus annuus CoA + 1-palmitoyl-glycerol 3-phosphate
-
?
2.3.1.15 palmitoyl-CoA + glycerol 3-phosphate and lineoyl-CoA, best substrates Helianthus annuus CoA + 1-palmitoyl-glycerol 3-phosphate
-
?
2.3.1.15 stearoyl-CoA + glycerol 3-phosphate
-
Helianthus annuus CoA + 1-stearoyl-glycerol 3-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase
-
Helianthus annuus
2.3.1.15 GPAT
-
Helianthus annuus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 30
-
assay at Helianthus annuus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 7.5
-
-
Helianthus annuus
2.3.1.15 7.5
-
assay at and optimal pH Helianthus annuus