BRENDA - Enzyme Database show

Cloning and characterization of the DHDPS gene encoding the lysine biosynthetic enzyme dihydrodipocolinate synthase from Zizania latifolia (Griseb)

Kong, F.; Jiang, S.; Meng, X.; Song, C.; Shi, J.; Jin, D.; Jiang, S.; Wang, B.; Plant Mol. Biol. Rep. 27, 199-208 (2009)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.3.7
into pBI121 vector in order to obtain fusion construct of the DHDPS sequence with the reporter gene GUS transiently expressed in the onion epidermal cells by particle gun-mediated bombardment. DHDPS cDNA coding sequence subcloned into the pUC18 plasmid and expressed in Escherichia coli strain AT997, an auxotroph lacking DHDPS activity
Zizania latifolia
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.3.3.7
cytoplasm
-
Zizania latifolia
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
36000
-
sequence analysis
Zizania latifolia
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Zizania latifolia
Q1PDD5
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
leaf
rarely present
Zizania latifolia
-
4.3.3.7
meristem
high level
Zizania latifolia
-
4.3.3.7
additional information
panicle axe
Zizania latifolia
-
4.3.3.7
seed
high level
Zizania latifolia
-
4.3.3.7
stem
rarely present
Zizania latifolia
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
-
706245
Zizania latifolia
dihydrodipicolinate + H2O
-
-
-
?
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.3.3.7
Zizania latifolia
sequence analysis
-
6.24
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
into pBI121 vector in order to obtain fusion construct of the DHDPS sequence with the reporter gene GUS transiently expressed in the onion epidermal cells by particle gun-mediated bombardment. DHDPS cDNA coding sequence subcloned into the pUC18 plasmid and expressed in Escherichia coli strain AT997, an auxotroph lacking DHDPS activity
Zizania latifolia
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.3.3.7
cytoplasm
-
Zizania latifolia
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
36000
-
sequence analysis
Zizania latifolia
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.3.7
leaf
rarely present
Zizania latifolia
-
4.3.3.7
meristem
high level
Zizania latifolia
-
4.3.3.7
additional information
panicle axe
Zizania latifolia
-
4.3.3.7
seed
high level
Zizania latifolia
-
4.3.3.7
stem
rarely present
Zizania latifolia
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
-
706245
Zizania latifolia
dihydrodipicolinate + H2O
-
-
-
?
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.3.3.7
Zizania latifolia
sequence analysis
-
6.24
Expression
EC Number
Organism
Commentary
Expression
4.3.3.7
Zizania latifolia
high level expression is present in fast-growing tissue and reproductive tissue. The 5'-regulatory sequence of DHDPS contains a GT-1 box and a (CA)n element, which may play a role in regulating the expression of DHDPS
up
General Information
EC Number
General Information
Commentary
Organism
4.3.3.7
physiological function
DHDPS can recover the DHDPS-deleted mutant of Escherichia coli
Zizania latifolia
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.3.3.7
physiological function
DHDPS can recover the DHDPS-deleted mutant of Escherichia coli
Zizania latifolia
Expression (protein specific)
EC Number
Organism
Commentary
Expression
4.3.3.7
Zizania latifolia
high level expression is present in fast-growing tissue and reproductive tissue. The 5'-regulatory sequence of DHDPS contains a GT-1 box and a (CA)n element, which may play a role in regulating the expression of DHDPS
up