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Literature summary extracted from

  • Yu, X.; Chen, M.; Liu, C.
    Nucleocytoplasmic-localized acyltransferases catalyze the malonylation of 7-O-glycosidic (iso)flavones in Medicago truncatula (2008), Plant J., 55, 382-396.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.115 nutrition health-promoting neutraceutical Medicago truncatula

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.115 overexpression in Arabidopsis thaliana Medicago truncatula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.115 0.0063
-
 naringenin 7-O-neohesperidoside
-
 Medicago truncatula
2.3.1.115 0.0078
-
 naringenin 7-O-neohesperidoside
-
 Medicago truncatula
2.3.1.115 0.0111
-
 malonyl-CoA
-
 Medicago truncatula
2.3.1.115 0.028
-
 naringenin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 0.0422
-
 malonyl-CoA
-
 Medicago truncatula
2.3.1.115 0.0512
-
 formononetin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 0.0713
-
 naringenin 7-O-neohesperidoside
-
 Medicago truncatula
2.3.1.115 0.1004
-
 naringenin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 0.114
-
 genistin
-
 Medicago truncatula
2.3.1.115 0.1148
-
 formononetin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 0.1256
-
 naringenin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 0.1314
-
 genistin
-
 Medicago truncatula
2.3.1.115 0.3094
-
 genistin
-
 Medicago truncatula
2.3.1.115 0.5539
-
 malonyl-CoA
-
 Medicago truncatula
2.3.1.115 0.736
-
 formononetin 7-O-glucoside
-
 Medicago truncatula

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.115 cytoplasm
-
 Medicago truncatula 5737
-
2.3.1.115 nucleus
-
 Medicago truncatula 5634
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.115 Medicago truncatula B4Y0U0
-
-
2.3.1.115 Medicago truncatula B4Y0U1
-
-
2.3.1.115 Medicago truncatula B4Y0U2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.115 leaf
-
 Medicago truncatula
-
2.3.1.115 root
-
 Medicago truncatula
-
2.3.1.115 stem
-
 Medicago truncatula
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.115 0.0017
-
-
 Medicago truncatula
2.3.1.115 0.0058
-
-
 Medicago truncatula
2.3.1.115 0.05
-
-
 Medicago truncatula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.115 malonyl-CoA + 6,4'-dihydroxy-7-O-glucosyl isoflavone assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + cyanidin 3,5-O-di-beta-D-glucoside assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + daidzin assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + formononetin 7-O-glucoside assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + genistin assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + hyperoside assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + isoflavone assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + isoquercitrin assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + keracyanin assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + kuromanin assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + naringenin 7-O-glucoside assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + naringenin 7-O-neohesperidoside assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?
2.3.1.115 malonyl-CoA + rutin assay at pH 8.0, 30°C Medicago truncatula ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.115 malonyl CoA:isoflavone 7-O-glucoside-6''-O-malonyltransferase
-
 Medicago truncatula
2.3.1.115 MtMaT1
-
 Medicago truncatula
2.3.1.115 MtMaT2
-
 Medicago truncatula
2.3.1.115 MtMaT3
-
 Medicago truncatula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.115 30
-
 assay at Medicago truncatula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.115 7.5
-
-
 Medicago truncatula
2.3.1.115 8
-
-
 Medicago truncatula

Expression

EC Number Organism Comment Expression
2.3.1.115 Medicago truncatula decreased expression by copper treatment down
2.3.1.115 Medicago truncatula decreased expression by drought stress down
2.3.1.115 Medicago truncatula high expression in root and leaf, induced expression by copper treatment, slightly induced expression after elicitation of a root cell suspension culture by yeast elicitor up
2.3.1.115 Medicago truncatula induced expression by drought stress and fungal infection up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.115 0.08
-
 genistin
-
 Medicago truncatula
2.3.1.115 0.0906
-
 genistin
-
 Medicago truncatula
2.3.1.115 0.1152
-
 formononetin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 0.2308
-
 malonyl-CoA
-
 Medicago truncatula
2.3.1.115 0.3365
-
 formononetin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 0.6249
-
 naringenin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 0.948
-
 naringenin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 1.01
-
 naringenin 7-O-neohesperidoside
-
 Medicago truncatula
2.3.1.115 1.52
-
 formononetin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 1.679
-
 naringenin 7-O-neohesperidoside
-
 Medicago truncatula
2.3.1.115 3.2
-
 naringenin 7-O-neohesperidoside
-
 Medicago truncatula
2.3.1.115 3.89
-
 naringenin 7-O-glucoside
-
 Medicago truncatula
2.3.1.115 3.9
-
 malonyl CoA
-
 Medicago truncatula
2.3.1.115 3.952
-
 malonyl-CoA
-
 Medicago truncatula
2.3.1.115 6.57
-
 genistin
-
 Medicago truncatula