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Literature summary extracted from

  • Stehle, F.; Brandt, W.; Stubbs, M.T.; Milkowski, C.; Strack, D.
    Sinapoyltransferases in the light of molecular evolution (2009), Phytochemistry, 70, 1652-1662.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.92 D172E/S175A mutant, relative activity 15% Arabidopsis thaliana
2.3.1.92 D358A mutant, relative activity 20% Arabidopsis thaliana
2.3.1.92 H411A mutant, no activity Arabidopsis thaliana
2.3.1.92 N73A mutant, relative activity 13% Arabidopsis thaliana
2.3.1.92 R322E mutant, relative activity 1% Arabidopsis thaliana
2.3.1.92 S173A mutant, no activity Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.92 (R)-3-hydroxybutyrate competitive inhibition of 13% Arabidopsis thaliana
2.3.1.92 (S)-2-hydroxybutyrate competitive inhibition of 12% Arabidopsis thaliana
2.3.1.92 D-malate competitive inhibition of 7% Arabidopsis thaliana
2.3.1.92 Glutarate competitive inhibition of 15% Arabidopsis thaliana
2.3.1.92 succinate competitive inhibition of 21% Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.92 0.115
-
 1-O-sinapoyl-beta-D-glucose
-
 Arabidopsis thaliana
2.3.1.92 0.89
-
 L-malate
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.92 1-O-sinapoyl-beta-D-glucose + L-malate Arabidopsis thaliana
-
 sinapoyl-L-malate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.92 Arabidopsis thaliana Q8RUW5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.92 0.0000084
-
 mutant R322E Arabidopsis thaliana
2.3.1.92 0.0001092
-
 mutant N73A Arabidopsis thaliana
2.3.1.92 0.000126
-
 mutant D172E/S175A Arabidopsis thaliana
2.3.1.92 0.000168
-
 mutant D358A Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.92 1-O-sinapoyl-beta-D-glucose + L-malate
-
 Arabidopsis thaliana sinapoyl-L-malate + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.92 1-O-sinapoyl-beta-glucose:L-malate sinapoyltransferase
-
 Arabidopsis thaliana
2.3.1.92 AtSMT
-
 Arabidopsis thaliana
2.3.1.92 SMT
-
 Arabidopsis thaliana