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Literature summary extracted from

  • Kaimoyo, E.; VanEtten, H.
    Inactivation of pea genes by RNAi supports the involvement of two similar O-methyltransferases in the biosynthesis of (+)-pisatin and of chiral intermediates with a configuration opposite that found in (+)-pisatin (2008), Phytochemistry, 69, 76-87.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.270
-
 Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone Pisum sativum
-
 S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain Pisum sativum HMM2 is involved primarily in the biosynthesis of (+)-pisatin S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.212 Pisum sativum
-
-
-
2.1.1.270 Pisum sativum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.270 root
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone
-
 Pisum sativum S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?
2.1.1.270 additional information in Pisum sativum, there are two highly similar hydroxymaackiain 3-O-methyltransferases HMM, with HMM1 prefering 2,7,4'-trihydroxyisoflavanone as a substrate and HMM2 being the specific O-methyltransferase that methylates (+)-6a-hydroxymaackiain Pisum sativum ?
-
 ?
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain isoform HMM2, 6a-hydroxymaackiain is the preferred substrate Pisum sativum S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?
2.1.1.270 S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain HMM2 is involved primarily in the biosynthesis of (+)-pisatin Pisum sativum S-adenosyl-L-homocysteine + (+)-pisatin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.270 6a-HMK methyltransferase
-
 Pisum sativum
2.1.1.270 HMM2
-
 Pisum sativum

General Information

EC Number General Information Comment Organism
2.1.1.270 physiological function hairy roots containing RNAi constructs of HMM are deficient in (+)-pisatin biosynthesis, and accumulate 2,7,4'-trihydroxyisoflavanone, daidzein, isoformononetin, and liquiritigenin. Accumulation of compounds is consistent with the blockage of the synthesis of (+)-pisatin at the HI4'OMT catalyzed step resulting in the accumulation of liquiritigenin and 2,7,4'-trihydroxyisoflavanone and the diversion of the pathway to produce daidzein and isoformononetin Pisum sativum