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Literature summary extracted from

  • Lang, I.; Feussner, I.
    Oxylipin formation in Nostoc punctiforme (PCC73102) (2007), Phytochemistry, 68, 1120-1127.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.12 expression in Escherichia coli Nostoc punctiforme

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.12 70891
-
 x * 70891, calculated from sequence Nostoc punctiforme
1.13.11.33 70891
-
 x * 70891, calculated from sequence Nostoc punctiforme

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.12 Nostoc punctiforme B2IZG6 PCC73102
-
1.13.11.33 Nostoc punctiforme B2IZG6 PCC73102
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.12 alpha-linolenate + O2 alpha-linolenate is the preferred substrate Nostoc punctiforme (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate
-
 ?
1.13.11.12 gamma-linolenate + O2
-
 Nostoc punctiforme (6Z,9Z,11E,13S)-13-hydroperoxy-6,9,11-octadecatrienoate
-
 ?
1.13.11.12 linoleate + O2
-
 Nostoc punctiforme (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate the ratio of (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate to (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate is higher than 80:20 at all pH values tested ?
1.13.11.12 additional information the enzyme also converts arachidonate to its (15S)-hydroperoxide. The enzyme shows no substrate preference Nostoc punctiforme ?
-
 ?
1.13.11.33 arachidonate + O2
-
 Nostoc punctiforme (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
 ?
1.13.11.33 additional information the enzyme also converts linoleate to (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate, alpha-linolenate to (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate and gamma-linolenate to (6Z,9Z,11E,13S)-13-hydroperoxy-6,9,11-octadecatrienoate Nostoc punctiforme ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.12 ? x * 70891, calculated from sequence Nostoc punctiforme
1.13.11.33 ? x * 70891, calculated from sequence Nostoc punctiforme

Synonyms

EC Number Synonyms Comment Organism
1.13.11.12 linoleate 13-lipoxygenase
-
 Nostoc punctiforme

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.12 4.5 8.5 broad optimum with a maximum at pH 8.0, substrate: linoleate Nostoc punctiforme