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Literature summary extracted from

  • Nagashima, S.; Hirotani, M.; Yoshikawa, T.
    Purification and characterization of UDP-glucuronate: baicalein 7-O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures (2000), Phytochemistry, 53, 533-538.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.253 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid 0.1 mM, complete inhibition Scutellaria baicalensis
2.4.1.253 CuSO4 1 mM, complete inhibition Scutellaria baicalensis
2.4.1.253 diethylpyrocarbonate 0.1 mM, 31% inhibition Scutellaria baicalensis
2.4.1.253 EDTA 5 mM, 17% inhibition Scutellaria baicalensis
2.4.1.253 FeCl2 1 mM, complete inhibition Scutellaria baicalensis
2.4.1.253 MgCl2 1 mM, 21.5% inhibition Scutellaria baicalensis
2.4.1.253 additional information N-ethylmaleimide has no effect in activity Scutellaria baicalensis
2.4.1.253 p-chloromercuribenzoate 0.1 mM, complete inhibition Scutellaria baicalensis
2.4.1.253 ZnCl2 1 mM, complete inhibition Scutellaria baicalensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.253 0.0524
-
 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one pH 7.5, 37°C Scutellaria baicalensis
2.4.1.253 0.0729
-
 UDP-glucuronate pH 7.5, 37°C Scutellaria baicalensis
2.4.1.253 0.107
-
 wogonin pH 7.5, 37°C Scutellaria baicalensis
2.4.1.253 0.109
-
 scutellarein pH 7.5, 37°C Scutellaria baicalensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.253 additional information no divalent cation requirement Scutellaria baicalensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.253 52000
-
 2 * 52000, does not exist or function as monomer, SDS-PAGE Scutellaria baicalensis
2.4.1.253 110000
-
 gel filtration Scutellaria baicalensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.253 UDP-D-glucuronate + 5,6,7-trihydroxyflavone Scutellaria baicalensis i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide ?
2.4.1.253 UDP-D-glucuronate + 5,6,7-trihydroxyflavone Scutellaria baicalensis Georgi i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.253 Scutellaria baicalensis
-
 Georgi
-
2.4.1.253 Scutellaria baicalensis Georgi
-
 Georgi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.253
-
 Scutellaria baicalensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.253 callus callus tissue derived from the petiole of a sterile young plant Scutellaria baicalensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.253 13.5
-
-
 Scutellaria baicalensis

Storage Stability

EC Number Storage Stability Organism
2.4.1.253 4°C, stable for more than 2 weeks without loss of activity Scutellaria baicalensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.253 additional information flavonoids lacking a substitution at the position ortho- to the 7-OH (chrysin, apigenin, luteolin, quercetin, formononetin and daidzein) do not serve as substrates Scutellaria baicalensis ?
-
 ?
2.4.1.253 additional information flavonoids lacking a substitution at the position ortho- to the 7-OH (chrysin, apigenin, luteolin, quercetin, formononetin and daidzein) do not serve as substrates Scutellaria baicalensis Georgi ?
-
 ?
2.4.1.253 UDP-D-glucuronate + 4',5,6,7-tetrahydroxyflavone i.e. 5,6,7-trihydroxy-2-(4-hydroxyphenyl)chromen-4-one, i.e. scutellarein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) Scutellaria baicalensis UDP + 4',5,6,7-tetrahydroxyflavone 7-O-beta-D-glucuronate i.e. scutellarein 7-O-glucuronide ?
2.4.1.253 UDP-D-glucuronate + 4',5,6,7-tetrahydroxyflavone i.e. 5,6,7-trihydroxy-2-(4-hydroxyphenyl)chromen-4-one, i.e. scutellarein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) Scutellaria baicalensis Georgi UDP + 4',5,6,7-tetrahydroxyflavone 7-O-beta-D-glucuronate i.e. scutellarein 7-O-glucuronide ?
2.4.1.253 UDP-D-glucuronate + 5,6,7-trihydroxyflavone i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) Scutellaria baicalensis UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide ?
2.4.1.253 UDP-D-glucuronate + 5,6,7-trihydroxyflavone i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) Scutellaria baicalensis UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide. Identification and quantitation of the enzyme reaction products are carried out by reversed phase-HPLC ?
2.4.1.253 UDP-D-glucuronate + 5,6,7-trihydroxyflavone i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) Scutellaria baicalensis Georgi UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide ?
2.4.1.253 UDP-D-glucuronate + 5,6,7-trihydroxyflavone i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) Scutellaria baicalensis Georgi UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide. Identification and quantitation of the enzyme reaction products are carried out by reversed phase-HPLC ?
2.4.1.253 UDP-D-glucuronate + 5,7-dihydroxy-8-methoxyflavone i.e. 5,7-dihydroxy-8-methoxy-2-phenylchromen-4-one, i.e. wogonin. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) Scutellaria baicalensis UDP + 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate i.e wogonin 7-O-glucuronide ?
2.4.1.253 UDP-D-glucuronate + 5,7-dihydroxy-8-methoxyflavone i.e. 5,7-dihydroxy-8-methoxy-2-phenylchromen-4-one, i.e. wogonin. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) Scutellaria baicalensis Georgi UDP + 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate i.e wogonin 7-O-glucuronide ?
2.4.1.253 UDP-glucuronate + baicalein
-
 Scutellaria baicalensis UDP + baicalin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.253 homodimer 2 * 52000, does not exist or function as monomer, SDS-PAGE Scutellaria baicalensis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.253 UBGAT
-
 Scutellaria baicalensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.253 30 40
-
 Scutellaria baicalensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.253 23
-
 50% of the activity remains after 3 h, 25% of the activity remains after 24 h Scutellaria baicalensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.253 7.5
-
 in 50 mM Tris-HCl buffer Scutellaria baicalensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.253 Scutellaria baicalensis chromatofocusing
-
 4.8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.253 additional information
-
 additional information Vmax/Km ratio for 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one (i.e. baicalein) is: 4.9 nkat/mg*micromol, Vmax/Km ratio for scutellarein is 2.67 nkat/mg*micromol, Vmax/Km ratio for wogonin is: 1.71 nkat/mg*micromol Scutellaria baicalensis