EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.31 | 13-hydroperoxy-octadecadienoic acid | - |
Homo sapiens | |
1.13.11.33 | 13-hydroperoxy-octadecadienoic acid | - |
Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.12 | Escherichia coli BL21 cells carrying the plasmid PT7-7/L1 VTSLO-11 | Glycine max |
1.13.11.31 | expressed with hexa-His tags | Homo sapiens |
1.13.11.33 | expressed with hexa-His tags | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.12 | 11-thialinoleic acid | is a competitive inhibitor with respect to linoleic acid and a noncompetitive inhibitor with respect to arachidonic acid | Glycine max | |
1.13.11.12 | 14-thialinoleic acid | is a competitive inhibitor with respect to linoleic acid | Glycine max | |
1.13.11.12 | additional information | presence of inhibitor does not change the regioselectivity of lipoxygenase-1 | Glycine max | |
1.13.11.31 | 11-thialinoleic acid | is a competitive inhibitor of 12-lipoxygenase with arachidonate as substrate. Presence of inhibitor does not alter the product distribution for 12-lipoxygenase. It does not change the regioselectivity of 12-lipoxygenase | Homo sapiens | |
1.13.11.33 | 11-thialinoleic acid | is a noncompetitive inhibitor of 15-lipoxygenase-1 with respect to arachidonate or linoleic acid as substrates. Presence of inhibitor does not alter the product distribution for 15-lipoxygenase-1. It does not change the regioselectivity of 15-lipoxygenase-1 | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.12 | 0.028 | - |
linoleic acid | - |
Glycine max | |
1.13.11.33 | 0.005 | - |
arachidonate | - |
Homo sapiens | |
1.13.11.33 | 0.0062 | - |
linoleic acid | - |
Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.31 | Fe | - |
Homo sapiens | |
1.13.11.33 | Fe | - |
Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.12 | Glycine max | - |
- |
- |
1.13.11.31 | Homo sapiens | - |
- |
- |
1.13.11.33 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.12 | by sonication, centrifugation and gel filtration | Glycine max |
1.13.11.31 | by one step Ni2+ affinity chromatography, more than 90% pure | Homo sapiens |
1.13.11.33 | by one step Ni2+ affinity chromatography, more than 90% pure | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.31 | blood platelet | - |
Homo sapiens | - |
1.13.11.33 | reticulocyte | - |
Homo sapiens | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.13.11.12 | -80°C, 100 mM borate buffer, pH 10, 30% glycerol | Glycine max |
1.13.11.31 | -80°C, 10-20% glycerol | Homo sapiens |
1.13.11.33 | -80°C, 10-20% glycerol | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.12 | linoleic acid + O2 | - |
Glycine max | (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid | - |
? | |
1.13.11.31 | arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | - |
? | |
1.13.11.33 | arachidonate + O2 | - |
Homo sapiens | (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyicosa-6,8,11,14-tetraenoate | - |
? | |
1.13.11.33 | linoleate + O2 | - |
Homo sapiens | (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.12 | lipoxygenase-1 | - |
Glycine max |
1.13.11.12 | LO-1 | - |
Glycine max |
1.13.11.31 | 12-lipoxygenase | - |
Homo sapiens |
1.13.11.31 | 12/15-LO | - |
Homo sapiens |
1.13.11.33 | 12/15-LO | - |
Homo sapiens |
1.13.11.33 | 15-lipoxygenase-1 | - |
Homo sapiens |
1.13.11.33 | 15-LO-1 | - |
Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.12 | 10 | - |
- |
Glycine max |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.12 | 0.022 | - |
11-thialinoleic acid | - |
Glycine max | |
1.13.11.12 | 0.035 | - |
14-thialinoleic acid | - |
Glycine max | |
1.13.11.31 | 0.0025 | - |
11-thialinoleic acid | - |
Homo sapiens |