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Literature summary extracted from

  • Tomikawa, C.; Yokogawa, T.; Kanai, T.; Hori, H.
    N7-methylguanine at position 46 (m7G46) in tRNA from Thermus thermophilus is required for cell viability at high temperatures through a tRNA modification network (2010), Nucleic Acids Res., 38, 942-957.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.33 expressed in Escherichia coli strain BL21 (DE3) Rosetta 2 Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA Thermus thermophilus
-
 S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.33 Thermus thermophilus
-
 strain HB8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.33 partially purified by heat treatment and DE52 column chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.33 S-adenosyl-L-methionine + guanine46 in tRNA
-
 Thermus thermophilus S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.33 TrmB
-
 Thermus thermophilus
2.1.1.33 TrMet (m7G46)
-
 Thermus thermophilus
2.1.1.33 tRNA (m7G46) methyltransferase
-
 Thermus thermophilus

General Information

EC Number General Information Comment Organism
2.1.1.33 malfunction protein synthesis in DELTAtrmB cells is depressed above 70°C. At 80°C, the DtrmB strain exhibits a severe growth defect Thermus thermophilus
2.1.1.33 physiological function the m7G46 modification catalyzed by TrmB may act as one of the key factors in tRNA modification network Thermus thermophilus
2.1.1.33 physiological function the m7G46 modification is required for cell viability at high temperatures via a tRNA modification network. The m7G46 modification induces nearly full modification of several modified nucleotides such as Gm18 and m1G37 Thermus thermophilus