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Literature summary extracted from
- Formation of the conserved pseudouridine at position 55 in archaeal tRNA (2006), Nucleic Acids Res., 34, 4293-4301.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.25 | additional information | pfuCbf5 can modify tRNA uridine55, but not rRNA, in the absence of Gar1, Nop10 and L7Ae. The activity of pfuCbf5 toward tRNA uridine55 is enhanced in the presence of the accessory proteins, suggesting that a multi-protein Cbf5 complex may function in pseudouridine55 synthesis in Pyrococcus furiosus | Pyrococcus furiosus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.25 | - |
Pyrococcus furiosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.25 | Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.25 | - |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.25 | tRNA uridine55 | assay with T7-transcribed pfutRNAAsp. Two distinct pseudouridine synthases specifically modify uridine55 in tRNA in vitro: 1. Cbf5, a protein known to play a role in RNA-guided modification of rRNA, and 2. pfuPus10 is not a member of the TruB/Pus4/Cbf5 family of pseudouridine synthases. Pus10 can pseudouridylate a truncated tRNA substrate and a tRNA lacking the 3'CCA. Cbf5 functions only on the full-length tRNA substrate including the 3'CCA end | Pyrococcus furiosus | tRNA pseudouridine55 | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.25 | Cbf5 | - |
Pyrococcus furiosus |
| 5.4.99.25 | pfuCbf5 | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.25 | 70 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.25 | 50 | 80 | about 50% of maximal activity at 50°C and at 80°C, pseudouridine synthase Cbf5 | Pyrococcus furiosus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.25 | evolution | the finding that in archaea Cbf5 may function in tRNA uridine55 pseudouridylation as well as RNA-guided pseudouridylation of rRNA supports the idea that Cbf5 is a direct descendant of a primordial TruB/Pus4-like tRNA PSI synthase | Pyrococcus furiosus |
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