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Literature summary extracted from

  • Gibellini, F.; Hunter, W.N.; Smith, T.K.
    The ethanolamine branch of the Kennedy pathway is essential in the bloodstream form of Trypanosoma brucei (2009), Mol. Microbiol., 73, 826-843.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.14 His-tagged version expressed in Escherichia coli BL21(DE3) Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.14 0.01209
-
 ethanolamine-phosphate
-
 Trypanosoma brucei
2.7.7.14 0.01267
-
 CTP
-
 Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.14 cytosol
-
 Trypanosoma brucei 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.14 44623
-
 1 * 44623, MALDI MS, analytical ultracentrifugation Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.14 Trypanosoma brucei B9WNA0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.14 Ni ion affinity chromatography Trypanosoma brucei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.14 1.55
-
-
 Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.14 CTP + ethanolamine phosphate
-
 Trypanosoma brucei CDP-ethanolamine + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.14 monomer 1 * 44623, MALDI MS, analytical ultracentrifugation Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.7.7.14 ethanolamine-phosphate cytidylyltransferase
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.14 1.15
-
 CTP
-
 Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.14 7.5
-
-
 Trypanosoma brucei

General Information

EC Number General Information Comment Organism
2.7.7.14 metabolism important for Kennedy pathway, essential to bloodstream form Trypanosoma brucei