Literature summary extracted from
Kim, T.G.; Kwon, T.H.; Min, K.; Dong, M.S.; Park, Y.I.; Ban, C.
Crystal structures of substrate and inhibitor complexes of ribose 5-phosphate isomerase A from Vibrio vulnificus YJ016 (2009), Mol. Cells, 27, 99-103.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.3.1.6 |
gene rpiA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) |
Vibrio vulnificus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.3.1.6 |
open form RpiA in complex with substrate ribose 5-phosphate, the closed form complexed with arabinose-5-phosphate, and the apo-RpiA, hanging drop vapor diffusion method, 0.002 ml of 30 mg/ml protein in 20 mM HEPES, pH 7.5, and 150 mM KCl are mixed with 0.002 ml of a reservoir solution containing 50 mM succinate, pH 4.1, 180 mM ammonium sulfate, and 8% PEG 4000, 3 days, 20°C, for complexed enzyme addition of 20 mM ligand, X-ray diffraction structure determination and analysis at 1.49-2.07 A resolution, molecular replacement |
Vibrio vulnificus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.3.1.6 |
arabinose 5-phosphate |
RpiA binds arabinose 5-phosphate located at the opening of the active site, the sugar ring of the inhibitor interacts with the Asp4, Lys7, Ser30, Asp118, and Lys121 residues, the phosphate group of arabinose 5-phosphate interacts with two water molecules, W51 and W82, binding mode of inhibitor, overview |
Vibrio vulnificus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.3.1.6 |
45000 |
- |
recombinant detagged RpiA, dynamic light scattering and gel filtration |
Vibrio vulnificus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
5.3.1.6 |
D-ribose 5-phosphate |
Vibrio vulnificus |
- |
D-ribulose 5-phosphate |
- |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.3.1.6 |
Vibrio vulnificus |
Q7MHL9 |
gene rpiA |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.3.1.6 |
recombinant His-tagged RpiA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography. The His-tag is cleaved off and removed, folowed by anion exchange chromatography and gel filtration |
Vibrio vulnificus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.3.1.6 |
D-ribose 5-phosphate |
- |
Vibrio vulnificus |
D-ribulose 5-phosphate |
- |
r |
|
5.3.1.6 |
D-ribose 5-phosphate |
binding mode of substrate, overview |
Vibrio vulnificus |
D-ribulose 5-phosphate |
- |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.3.1.6 |
dimer |
2 * 20000-25000, RpiA |
Vibrio vulnificus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.3.1.6 |
ribose 5-phosphate isomerase A |
- |
Vibrio vulnificus |
5.3.1.6 |
RpiA |
- |
Vibrio vulnificus |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
5.3.1.6 |
Vibrio vulnificus |
isoelectric focusing |
- |
4.77 |