EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.3.4 | expressed as His-tagged recombinant protein in Escherichia coli pTrcAtuE cells | Pseudomonas aeruginosa |
4.1.3.26 | His-tagged protein expressed in Escherichia coli JM101 | Pseudomonas aeruginosa PAO1 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.4 | 33000 | - |
LiuE His-tagged protein, SDS-PAGE | Pseudomonas aeruginosa |
4.1.3.26 | 33000 | - |
x * 33000, SDS-PAGE | Pseudomonas aeruginosa PAO1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.4 | Pseudomonas aeruginosa | - |
- |
- |
4.1.3.4 | Pseudomonas aeruginosa PAO1SM | - |
- |
- |
4.1.3.26 | Pseudomonas aeruginosa PAO1 | Q9I2A0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.26 | by affinity chromatography, more than 98% purified | Pseudomonas aeruginosa PAO1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.4 | (S)-3-Hydroxy-3-methylglutaryl-CoA | - |
Pseudomonas aeruginosa | Acetyl-CoA + acetoacetate | - |
? | |
4.1.3.4 | (S)-3-Hydroxy-3-methylglutaryl-CoA | - |
Pseudomonas aeruginosa PAO1SM | Acetyl-CoA + acetoacetate | - |
? | |
4.1.3.26 | 3-hydroxy-3-isohexenylglutaryl-CoA | - |
Pseudomonas aeruginosa PAO1 | 7-methyl-3-oxo-6-octenyl-CoA + acetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.3.26 | ? | x * 33000, SDS-PAGE | Pseudomonas aeruginosa PAO1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.3.4 | 3-hydroxy-3-methylglutaryl CoA lyase | - |
Pseudomonas aeruginosa |
4.1.3.4 | HMG-CoA lyase | - |
Pseudomonas aeruginosa |
4.1.3.4 | LiuE protein | in addition to show HMG-CoA lyase activity, LiuE also displays 3-hydroxy-3-isohexenylglutaryl-CoA lyase activity | Pseudomonas aeruginosa |
4.1.3.26 | 3-hydroxy-3-isohexenylglutaryl-CoA lyase | - |
Pseudomonas aeruginosa PAO1 |
4.1.3.26 | HIHG-CoA lyase | - |
Pseudomonas aeruginosa PAO1 |
4.1.3.26 | LiuE | - |
Pseudomonas aeruginosa PAO1 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.3.26 | metabolism | LiuE displays a bifunctional role in both the leucine/isovalerate and acyclic terpenes catabolic pathways, LiuE shows enzymatic activity of HMG-CoA lyase and also HIHG-CoA lyase activity | Pseudomonas aeruginosa PAO1 |