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Literature summary extracted from

  • Maenpuen, S.; Sopitthummakhun, K.; Yuthavong, Y.; Chaiyen, P.; Leartsakulpanich, U.
    Characterization of Plasmodium falciparum serine hydroxymethyltransferase-A potential antimalarial target (2009), Mol. Biochem. Parasitol., 168, 63-73.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.1 medicine SHMT is a potential target for antimalarial chemotherapy Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 His6-tagged enzyme is expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Plasmodium falciparum

General Stability

EC Number General Stability Organism
2.1.2.1 the kcat value of the enzyme in phosphate buffer is about 60% of that measured in HEPES buffer Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 glycine glycine inhibits His6-tagged SHMT competitively with respect to serine and non-competitively with respect to tetrahydrofolate Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.071
-
 D-serine apparent value, in 50 mM HEPES, pH 8.0 containing 0.5 mM EDTA, 1 mM dithiothreitol, at 25°C Plasmodium falciparum
2.1.2.1 0.193
-
 tetrahydrofolate in 50 mM HEPES buffer, pH 7.45, at 25°C Plasmodium falciparum
2.1.2.1 0.251
-
 L-serine in phosphate buffer, pH 7.45, at 25°C Plasmodium falciparum
2.1.2.1 0.253
-
 tetrahydrofolate in phosphate buffer, at 25°C Plasmodium falciparum
2.1.2.1 0.371
-
 L-serine in 50 mM HEPES buffer, pH 7.45, at 25°C Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 55000
-
 2 * 55000, calculated from amino acid sequence Plasmodium falciparum
2.1.2.1 85000
-
 native enzyme, SDS-PAGE Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Plasmodium falciparum
-
 strainTM4
-
2.1.2.1 Plasmodium falciparum TM4
-
 strainTM4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 Ni-Sepharose column chromatography, Sephadex G-25 gel filtration, and SP-Sepharose column chromatography Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 D-serine + tetrahydrofolate the catalytic efficiency for D-serine is 580fold lower than that of L-serine Plasmodium falciparum glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 D-serine + tetrahydrofolate the catalytic efficiency for D-serine is 580fold lower than that of L-serine Plasmodium falciparum TM4 glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 L-serine + tetrahydrofolate only (6S)-tetrahydrofolate can serve as a substrate for His6-tagged SHMT, the presence of (6R)-tetrahydrofolate has no interference to the reaction Plasmodium falciparum glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.1 L-serine + tetrahydrofolate only (6S)-tetrahydrofolate can serve as a substrate for His6-tagged SHMT, the presence of (6R)-tetrahydrofolate has no interference to the reaction Plasmodium falciparum TM4 glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 homodimer 2 * 55000, calculated from amino acid sequence Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Plasmodium falciparum
2.1.2.1 SHMT
-
 Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 0.246
-
 D-serine apparent value, in 50 mM HEPES, pH 8.0 containing 0.5 mM EDTA, 1 mM dithiothreitol, at 25°C Plasmodium falciparum
2.1.2.1 0.43
-
 L-serine in phosphate buffer, pH 7.45, at 25°C Plasmodium falciparum
2.1.2.1 0.74
-
 L-serine in 50 mM HEPES buffer, pH 7.45, at 25°C Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.2.1 0.00346
-
 D-serine apparent value, in 50 mM HEPES, pH 8.0 containing 0.5 mM EDTA, 1 mM dithiothreitol, at 25°C Plasmodium falciparum
2.1.2.1 1.99
-
 L-serine in 50 mM HEPES buffer, pH 7.45, at 25°C Plasmodium falciparum